{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:44:41Z","timestamp":1785761081947,"version":"3.56.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52522215"],"award-info":[{"award-number":["52522215"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52272275"],"award-info":[{"award-number":["52272275"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cellular pyroptosis, a pro\u2010inflammatory form of programmed cell death, emerges as a promising strategy for cancer immunotherapy. However, gene methylation hampers the expression of gasdermin E (GSDME), a pivotal protein in the caspase\u20103\u2010mediated pyroptosis pathway, across numerous malignancies, including triple\u2010negative breast cancer (TNBC). This study elucidated that a colloidal hydrogel (GDLC hydrogel) loaded with decitabine (DAC) and crosslinked with lanthanum carbonate nanosheets (LCNSs) and gelatin nanoparticles (Gela NPs) could instigate pyroptosis in TNBC cells, specifically 4T1 cells. Within the tumor microenvironment (TME), the synergistic interaction between DAC and La\n                    <jats:sup>3+<\/jats:sup>\n                    reversed the epigenetic silencing of the GSDME gene, while concurrently activating caspase\u20103, thus facilitating pyroptosis induction. Although monotherapy with La\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010induced apoptosis proved insufficient in tumor suppression, the concurrent inhibition of methyltransferase via DAC markedly suppressed both primary and recurrent tumors. Moreover, the combinatory application of the GDLC hydrogel and immune checkpoint inhibitors effectively neutralized the immunosuppressive TME of TNBC, triggered an anti\u2010tumor immune response and engendered a sustained immune memory effect. This strategy significantly curtailed tumor recurrence and metastasis, consequently improving the survival rates of mice in the 4T1 tumor\u2010bearing mouse model. In conclusion, this colloidal hydrogel platform introduced an innovative paradigm for the immunotherapy of triple\u2010negative breast cancer.\n                  <\/jats:p>","DOI":"10.1002\/advs.76966","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T11:46:35Z","timestamp":1785757595000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lanthanum Carbonate Nanosheets Based Colloidal Hydrogel for Modulating Innate &amp; Adaptive Tumor Immunotherapy by Augmented Cellular Pyroptosis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7235-7994","authenticated-orcid":false,"given":"Yang","family":"Hong","sequence":"first","affiliation":[{"name":"School of Medicine and Health Zhengzhou Advanced Research Institute of Harbin Institute of Technology  Zhengzhou China"},{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8769-9190","authenticated-orcid":false,"given":"Haitao","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9257-0817","authenticated-orcid":false,"given":"Weitao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1173-021X","authenticated-orcid":false,"given":"Yaoyu","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3646-4969","authenticated-orcid":false,"given":"Zhengbao","family":"Zha","sequence":"additional","affiliation":[{"name":"School of Medicine and Health Zhengzhou Advanced Research Institute of Harbin Institute of Technology  Zhengzhou China"},{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"issue":"7","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.202311362","article-title":"Biomaterials Elicit Pyroptosis Enhancing Cancer Immunotherapy","volume":"34","author":"Zhang M.\u2010J.","year":"2024","journal-title":"Advanced Functional Materials"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.nantod.2023.102033","article-title":"Biological Effects of Metal\u2010Based Nanomaterials for Tumor Metalloimmunotherapy","volume":"53","author":"Lei H.","year":"2023","journal-title":"Nano Today"},{"issue":"1","key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1038\/s41392-022-01046-3","article-title":"Autophagy, Ferroptosis, Pyroptosis, and Necroptosis in Tumor Immunotherapy","volume":"7","author":"Gao W.","year":"2022","journal-title":"Signal Transduction and Targeted Therapy"},{"issue":"1","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/s41420-022-01101-6","article-title":"Emerging Mechanisms of Pyroptosis and Its Therapeutic Strategy in Cancer","volume":"8","author":"Lu L.","year":"2022","journal-title":"Cell Death Discovery"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"vi31","DOI":"10.1093\/annonc\/mdw363.50","article-title":"Large Scale DFNA5 Methylation and Expression Analysis in Primary Breast Adenocarcinoma Using Data from the Cancer Genome Atlas","volume":"27","author":"Croes L.","year":"2016","journal-title":"Annals of Oncology"},{"issue":"25","key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"3624","DOI":"10.1038\/sj.onc.1211021","article-title":"Aberrant Promoter Methylation and Tumor Suppressive Activity of the DFNA5 Gene in Colorectal Carcinoma","volume":"27","author":"Kim M. 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