{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:33:04Z","timestamp":1786465984352,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360185"],"award-info":[{"award-number":["32360185"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The efficacy of dendritic cell (DC) vaccines in cancer immunotherapy is often limited by weak immunogenicity due to inefficient antigen cross\u2010presentation (XPT). We previously combined a calcium carbonate (CaCO\n                    <jats:sub>3<\/jats:sub>\n                    ) delivery system with a proteolysis\u2010targeting chimera (PROTAC) to enhance antigen degradation. Along this line, we developed a protein\/DNA\u2010integrated nanoantigen platform (HpOAC) co\u2010loading CaCO\n                    <jats:sub>3<\/jats:sub>\n                    with a His\u2010tagged ovalbumin (O) modified with the von Hippel\u2010Lindau (VHL) E3 ligase\u2010recruiting peptide ALAPYIP (HOA) and a eukaryotic plasmid encoding O\u2010ALAPYIP (pOA). HpOAC promoted DC maturation and migration via Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release. Mechanistically, HOA provided an immediate antigen source, while pOA enabled sustained expression; both underwent ALAPYIP\u2010mediated VHL recruitment, enhancing O ubiquitination and proteasomal degradation, thereby amplifying XPT. Consequently, HpOAC\u2010DCs induced strong OT\u2010I\/OT\u2010II T cell proliferation in vitro. In vivo, a single immunization with HpOAC\u2010DCs significantly suppressed tumor growth and elicited durable antigen\u2010specific T helper type 1 and cytotoxic T lymphocyte responses, outperforming single\u2010antigen vaccines. Under a two\u2010dose regimen, its anti\u2010tumor efficacy was improved compared to non\u2010PROTAC and in situ antigen vaccines. Thus, we constructed a PROTAC\u2010enhanced, protein\/DNA\u2010integrated DC vaccine platform that synergizes immediate and sustained antigen supply with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010mediated adjuvanticity, offering a highly promising strategy for novel DC vaccine design.\n                  <\/jats:p>","DOI":"10.1002\/advs.77089","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T15:25:30Z","timestamp":1786461930000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["PROTAC\u2010Engineered Protein\/DNA Nanoantigen is a Potent Booster for Dendritic Cell Vaccines in Cancer Immunotherapy"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9100-4706","authenticated-orcid":false,"given":"Peng","family":"Liu","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3500-6634","authenticated-orcid":false,"given":"Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhou","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1100-3982","authenticated-orcid":false,"given":"Yuekang","family":"Xu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3295-6096","authenticated-orcid":false,"given":"Jinyao","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology Xinjiang University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541\u2010021\u201000330\u20107"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.140558"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2023.168297"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2021.03.027"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1108003"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010022\u201000707\u20102"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2026.2635774"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010020\u201001048\u20104"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.06.003"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201013438\u20101"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010023\u201001105\u2010x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028480"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.2.165"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201001270\u2010x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423\u2010023\u201000990\u20106"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010024\u201000859\u20101"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2014.10.020"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102025\u2010012405"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.115263"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043625\u20100"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c07306"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201704272"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001381\u20104"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010024\u201001813\u2010z"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00767"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059796"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059817"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2008.26"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c00550"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.09.025"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201054402\u2010y"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2014.03.007"},{"key":"e_1_2_10_34_1","first-page":"481","volume-title":"Calcium 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