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It is associated not only with periodontal disease but also with various systemic diseases, including Alzheimer's disease, rheumatoid arthritis, and atherosclerosis. Therefore, early detection of this bacterium is crucial for enabling subsequent treatment and prevention of such conditions. Herein, we report a chromogenic release\u2010based assay system utilizing calcium carbonate microspheres and a dye\u2010labeled peptide that is selectively cleaved by an Arg\u2010specific protease called gingipain (RgpB) that is secreted by\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    . By utilizing the reactive anhydride functional groups of poly(isobutylene\u2010\n                    <jats:italic>alt<\/jats:italic>\n                    \u2010maleic anhydride), we simultaneously achieved the conjugation of the dye\u2010labeled peptide and its immobilization onto calcium carbonate microspheres. Through optimization of the peptide sequence and the surface modification density on calcium carbonate microspheres, the assay of RgpB detection with the absorbance of supernatant exhibited a low limit of detection of 0.25\u00a0nM, as well as high storage stability and selectivity for\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    . The measurements obtained with this assay system for clinical gingival crevicular fluid samples showed a high correlation (Pearson's\n                    <jats:italic>r<\/jats:italic>\n                    = 0.79) with qPCR results. This assay system offers an on\u2010site, visual means to monitor\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    activity without requiring specialized instrumentation.\n                  <\/jats:p>","DOI":"10.1002\/advs.77102","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:25:26Z","timestamp":1787052326000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protease\u2010Triggered Chromogenic Release From Peptide\u2010Modified Calcium Carbonate Microspheres Enables Colorimetric Detection of\n                    <i>Porphyromonas gingivalis<\/i>\n                    Activity"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7303-4189","authenticated-orcid":false,"given":"Yuki","family":"Hiruta","sequence":"first","affiliation":[{"name":"Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California San Diego  California USA"},{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6072-7533","authenticated-orcid":false,"given":"Zhicheng","family":"Jin","sequence":"additional","affiliation":[{"name":"Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California San Diego  California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Adachi","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Molecular and Cell Biology University of California San Diego  California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5715-5060","authenticated-orcid":false,"given":"Lubna","family":"Amer","sequence":"additional","affiliation":[{"name":"Program in Materials Science and Engineering University of California San Diego  California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia O.","family":"Tjokro","sequence":"additional","affiliation":[{"name":"Herman Ostrow School of Dentistry University of Southern California  Los Angeles USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego F.","family":"Trujillo","sequence":"additional","affiliation":[{"name":"Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego  California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Biochemistry Biophysics and Biotechnology Jagiellonian University  Krakow Poland"},{"name":"Department of Oral Immunology and Infectious Diseases School of Dentistry University of Louisville  Louisville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5695-0409","authenticated-orcid":false,"given":"Anthony J.","family":"O'Donoghue","sequence":"additional","affiliation":[{"name":"Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego  California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casey","family":"Chen","sequence":"additional","affiliation":[{"name":"Herman Ostrow School of Dentistry University of Southern California  Los Angeles USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-6408","authenticated-orcid":false,"given":"Jesse V.","family":"Jokerst","sequence":"additional","affiliation":[{"name":"Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California San Diego  California USA"},{"name":"Program in Materials Science and Engineering University of California San Diego  California 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