{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T16:34:41Z","timestamp":1786638881848,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Organ transplantation provides a life\u2010saving intervention for patients with end\u2010stage lung disease; however, access to this treatment remains limited by the shortage of donor organs.\n                    <jats:italic>Ex vivo<\/jats:italic>\n                    lung perfusion (EVLP) is a platform that enables surgeons to assess extended\u2010criteria for donor lungs prior to transplantation and has safely expanded the donor pool. Yet current EVLP biochemical assessments rely on intermittent manual sampling and fail to capture dynamic metabolic changes. Here we present MetaboSense, a first\u2010of\u2010its\u2010kind microfluidic platform for continuous, real\u2010time, multiplexed monitoring of glucose and lactate\u2014key metabolites that report on donor lung metabolism\u2014during EVLP. The system integrates a bead\u2010based, quantum dot\u2010mediated aptamer assay with modular microfluidics for in\u2010line biomarker measurement. MetaboSense achieves limits of detection of 0.74\u00a0mM (glucose) and 1.19\u00a0mM (lactate) with analytical precision (R\n                    <jats:sup>2<\/jats:sup>\n                    &gt; 0.97). Clinical validation across eleven EVLP cases demonstrates strong correlation with gold\u2010standard arterial blood gas measurements. In\u2010line deployment within an EVLP circuit during perfusion of porcine lungs confirms MetaboSense's ability to track metabolite fluctuations with 60\u2010s temporal resolution. This platform eliminates labor\u2010intensive sampling and provides unprecedented granularity in lung metabolomics during EVLP, representing an important step toward real\u2010time EVLP analytics and improved organ selection and transplant outcomes.\n                  <\/jats:p>","DOI":"10.1002\/advs.77123","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:50:36Z","timestamp":1786636236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MetaboSense: An Integrated Quantum Dot\u2010Mediated Aptamer Assay and Modular Microfluidic Platform for Continuous In\u2010Line Monitoring of Lung Metabolism During\n                    <i>Ex Vivo<\/i>\n                    Perfusion"],"prefix":"10.1002","author":[{"given":"Sanjana","family":"Srikant","sequence":"first","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Waterloo  Waterloo Ontario Canada"},{"name":"Waterloo Institute For Nanotechnology University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0286-2848","authenticated-orcid":false,"given":"Pouyan","family":"Keshavarz Motamed","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Waterloo  Waterloo Ontario Canada"},{"name":"Waterloo Institute For Nanotechnology University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Borrillo","sequence":"additional","affiliation":[{"name":"Latner Thoracic Research Laboratories Toronto General Hospital University Health Network  Toronto Ontario Canada"},{"name":"Toronto Lung Transplant Program Ajmera Transplant Centre University Health Network  Toronto Ontario 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Al","family":"Fattah","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Waterloo  Waterloo Ontario Canada"},{"name":"Waterloo Institute For Nanotechnology University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Jaber","sequence":"additional","affiliation":[{"name":"Latner Thoracic Research Laboratories Toronto General Hospital University Health Network  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5271-3163","authenticated-orcid":false,"given":"Dayan","family":"Ban","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Waterloo  Waterloo Ontario Canada"},{"name":"Waterloo Institute For Nanotechnology University of Waterloo  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Cypel","sequence":"additional","affiliation":[{"name":"Latner Thoracic Research Laboratories Toronto General Hospital University Health Network  Toronto Ontario Canada"},{"name":"Toronto Lung Transplant Program Ajmera Transplant Centre University Health Network  Toronto Ontario Canada"},{"name":"Institute of Medical Science Temerty Faculty of Medicine University of Toronto  Toronto Ontario Canada"},{"name":"Department of Surgery Temerty Faculty of Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-8417","authenticated-orcid":false,"given":"Mingyao","family":"Liu","sequence":"additional","affiliation":[{"name":"Latner Thoracic Research Laboratories Toronto General Hospital University Health Network  Toronto Ontario Canada"},{"name":"Toronto Lung Transplant Program Ajmera Transplant Centre University Health Network  Toronto Ontario Canada"},{"name":"Institute of Medical Science Temerty Faculty of Medicine University of Toronto  Toronto Ontario Canada"},{"name":"Department of Surgery Temerty Faculty of Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaf","family":"Keshavjee","sequence":"additional","affiliation":[{"name":"Latner Thoracic Research Laboratories Toronto General Hospital University Health Network  Toronto Ontario Canada"},{"name":"Toronto Lung Transplant Program Ajmera Transplant Centre University Health Network  Toronto Ontario Canada"},{"name":"Institute of Medical Science Temerty Faculty of Medicine University of Toronto  Toronto Ontario Canada"},{"name":"Department of Surgery Temerty Faculty of Medicine University of Toronto  Toronto Ontario 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