{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:59:24Z","timestamp":1787324364285,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YEF0132500"],"award-info":[{"award-number":["2022YEF0132500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302653"],"award-info":[{"award-number":["82302653"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32101137"],"award-info":[{"award-number":["32101137"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770900"],"award-info":[{"award-number":["81770900"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Burns represent a complex and disabling global public health problem, presenting substantial clinical challenges attributed to disrupted tissue microenvironment and persistent wound infections, which impair the synergistic interaction between fibroblasts (Fb) and keratinocytes (KC). FGF2 and its homologues widely used for burns show weak wound\u2010repair efficacy, owing to poor targeting and degradation by bacteria and proteases. In this study, FGF2\u2010modified extracellular vesicles (F\u2010EVs) were engineered from human adipose mesenchymal stem cells (ADMSCs) via electrostatic adsorption, and further combined with 4D\u2010printed hydrogels to construct a 4D@F\u2010EVs system. This system was designed to reconstruct the burn tissue microenvironment, exert antibacterial effects, and restore the crosstalk between Fb and KC. In vitro and in vivo analysis confirmed that the 4D@F\u2010EVs were precisely delivered to Fb, promoting cell proliferation and facilitating the reconstruction of the burn tissue microenvironment. Single\u2010cell RNA sequencing (scRNA\u2010seq) analysis revealed that the regulatory effect of 4D@F\u2010EVs on skin regeneration involves the activation of the PI3K\u2010Akt signaling pathway and the promotion of crosstalk between Keratinocyte Growth Factor\u2010positive (KGF\n                    <jats:sup>+<\/jats:sup>\n                    ) Fb and Keratinocyte Growth Factor Receptor\u2010positive (KGFR\n                    <jats:sup>+)<\/jats:sup>\n                    KC. Collectively, this work offers an original clinical approach for infected burn wounds by reconstructing the impaired microenvironment and restoring intercellular crosstalk.\n                  <\/jats:p>","DOI":"10.1002\/advs.77141","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:22:30Z","timestamp":1787322150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application and Single\u2010Cell Regulation Mechanism of Engineered EVs Combined With 4D\u2010Printed Hydrogel for Infected Burn Repair"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3627-6936","authenticated-orcid":false,"given":"Xiaomin","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"},{"name":"School of Basic Medicine Qingdao University  Qingdao P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1387-7373","authenticated-orcid":false,"given":"Kelun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Cutaneous Biology Research Institute Severance Hospital Yonsei University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjiao","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"},{"name":"School of Basic Medicine Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Shang","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. 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R. China"},{"name":"School of Basic Medicine Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4791-1842","authenticated-orcid":false,"given":"Xiaotong","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taoyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Tao","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxu","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9326-6171","authenticated-orcid":false,"given":"Xiaoying","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7426-6950","authenticated-orcid":false,"given":"Wenhua","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine and Laboratory Technology Innovation Qingdao University  Qingdao Shandong P. R. 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Platelet\u2010Rich Plasma Treatment for Photoaged Facial Skin: An Investigator\u2010Blinded, Split\u2010Face, Non\u2010Inferiority Trial","volume":"24","author":"Estupi\u00f1an B.","year":"2025","journal-title":"Journal of Cosmetic Dermatology"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2025.01.007"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12993"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616\u2010025\u201000793\u20109"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201001263\u2010w"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ad1e6f"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612828666220519145859"},{"key":"e_1_2_12_45_1","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202420338","article-title":"Thrombin\u2010Anchored Bacterial Cellulose Dressing for Advanced Burn Wound 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