{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T16:29:34Z","timestamp":1786638574184,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82560293"],"award-info":[{"award-number":["82560293"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Heat shock triggers stress granule (SG) formation, yet their dynamics in mammalian germ cells remain unclear. Through systematic analysis, we uncovered distinct heat shock granule (HSG) composition and stage\u2010specific assembly\/disassembly in the mouse testis. Ribosome profiling revealed that germ cells selectively sustain translation of spermatogenic genes during heat shock. We identified that the germ cell\u2010specific RNA\u2010binding protein BOULE is essential for HSG clearance. BOULE deficiency under heat shock impairs HSG disassembly, with increased germ cell apoptosis and a disrupted ubiquitinome. Mechanistically, BOULE orchestrates HSG clearance through a two\u2010pronged mechanism. It post\u2010transcriptionally maintains the expression of the disassembly factors G3BP1 and FAF2. Additionally, BOULE promotes heat\u2010shock\u2010induced ubiquitination of G3BP1 by regulating the E3 ligase TRIM27, thereby recruiting the VCP\/FAF2 complex. Our findings establish BOULE as a key regulator of proteostasis that coordinates HSG disassembly in germ cells, providing mechanistic insight into heat shock\u2010induced germ cell damage that may contribute to male infertility.<\/jats:p>","DOI":"10.1002\/advs.77155","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:32:32Z","timestamp":1786635152000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5632-5427","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center Jiangxi Maternal and Child Health Hospital Nanchang Medical College  Nanchang Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center For Reproductive Medicine The Third Affiliated Hospital of Zhengzhou University  Zhengzhou Henan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexin","family":"Bian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health Nanjing Medical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baobao","family":"Geng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Common Mechanism Research for Major Diseases Suzhou Institute of Systems Medicine Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyun","family":"Cao","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Jiangxi Maternal and Child Health Hospital Nanchang Medical College  Nanchang Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Jiangxi Maternal and Child Health Hospital Nanchang Medical College  Nanchang Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengming","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Jiangxi Maternal and Child Health Hospital Nanchang Medical College  Nanchang Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Jiangxi Maternal and Child Health Hospital Nanchang Medical College  Nanchang Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9080-7534","authenticated-orcid":false,"given":"Jun","family":"Tan","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Jiangxi Maternal and Child Health Hospital Nanchang Medical College  Nanchang Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.tcb.2016.05.004","article-title":"Principles and Properties of Stress Granules","volume":"26","author":"Protter D. 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