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Herein, we developed an activatable near\u2010infrared fluorescent nanoprobe (denoted PS\u2010QRC) by encapsulating a self\u2010amplifying fluorescent sensor (QRCy) within kidney\u2010targetable\n                    <jats:italic>L<\/jats:italic>\n                    \u2010serine\u2010functionalized ultrasmall poly(amidoamine) (PAMAM\u2010Ser) dendrimers. Upon activation by reactive oxygen species (ROS), QRCy undergoes self\u2010fragmentation, releasing four fluorophores to amplify the fluorescence signal, affording an ultra\u2010low detection limit of 81\u00a0nM for hydrogen peroxide. The\n                    <jats:italic>L<\/jats:italic>\n                    \u2010serine modification enables specific binding of PS\u2010QRC to kidney injury molecule\u20101 (KIM\u20101), which is overexpressed in injured renal tubules, thereby promoting renal accumulation and reducing background interference. Impressively, PS\u2010QRC allows noninvasive real\u2010time imaging of cisplatin\u2010induced AKI in mice as early as 12\u00a0h, significantly earlier than conventional serum biochemical and histopathological analyses. Furthermore, a high\u2010throughput screening platform was established using this nanoprobe to evaluate natural products, from which chrysin was identified as a promising renoprotective agent. Chrysin demonstrated both in vitro and in vivo renoprotective effects, with efficacy comparable to the established antioxidant\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylcysteine. Moreover, PS\u2010QRC enabled quantitative monitoring of chrysin's dose\u2010dependent therapeutic response. Overall, this integrated probe provides a sensitive and reliable tool for early AKI diagnosis and the high\u2010throughput discovery of novel renoprotective drugs.\n                  <\/jats:p>","DOI":"10.1002\/advs.77162","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:53:45Z","timestamp":1786636425000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Self\u2010Amplified Activatable Fluorescent Probe for Early Diagnosis of Acute Kidney Injury and High\u2010Throughput Screening of Natural Protectants"],"prefix":"10.1002","author":[{"given":"Yahui","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Biomaterials of Guangdong Higher Education Institutes Department of Biomedical Engineering and MOE Key Laboratory of Tumor Molecular Biology Jinan University  Guangzhou People's Republic of 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