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Here, guided by structural insights into a compact Cas9d nuclease from\n                    <jats:italic>Deltaproteobacteria<\/jats:italic>\n                    , we developed an efficient Cas9d system (Cas9d\n                    <jats:sup>Ultra<\/jats:sup>\n                    ) through gRNA and protein engineering, and further developed its base editors (9dBEs). Cas9d\n                    <jats:sup>Ultra<\/jats:sup>\n                    and 9dBEs enabled efficient and precise genome editing in human cells. Notably, 9dCBE induced premature termination codons in 89% of mouse pups by microinjection, facilitating robust disease modeling. Furthermore, a single AAV delivering 9dCBE achieved efficient\n                    <jats:italic>Pcsk9<\/jats:italic>\n                    editing and a concomitant reduction in serum LDL\u2010C levels in mice. Collectively, this study establishes a series of compact, potent genome editing tools poised to advance biological and biomedical translational research.\n                  <\/jats:p>","DOI":"10.1002\/advs.77279","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T10:35:37Z","timestamp":1787135737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3921-9395","authenticated-orcid":false,"given":"Qingquan","family":"Xiao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Advanced Drug Delivery and Release Systems Jinhua Institute of Zhejiang University School of Pharmacy Zhejiang University  Hangzhou 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Zhejiang University School of Pharmacy Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoling","family":"Li","sequence":"additional","affiliation":[{"name":"Zhongshan Institute for Drug Discovery Shanghai Institute of Materia Medica Chinese Academy of Sciences  Zhongshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6642-1559","authenticated-orcid":false,"given":"Yinghui","family":"Wei","sequence":"additional","affiliation":[{"name":"International Joint Agriculture Research Center for Animal Bio\u2010Breeding of Ministry of Agriculture and Rural Affairs College of Animal Science and Technology Northwest A&amp;F University  Yangling China"},{"name":"Hainan Institute Northwest A&amp;F University  Sanya 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