{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T14:27:08Z","timestamp":1787149628037,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Murine models are essential for cardiovascular translational research for developing new therapies to reduce the risk of heart attacks. However, assessment of murine atherosclerotic plaques by traditional histological methods fails to capture the entirety of plaque development across the length of a blood vessel over time. Here, the authors have developed and validated the first longitudinal molecular and microstructural intravascular imaging approach to study murine atherosclerosis. This approach combines a unique murine surgical model and\u00a0a miniaturized multimodal intravascular imaging device with optical coherence tomography (OCT) and fluorescence imaging capabilities able to detect infused indocyanine green (ICG), a marker of macrophages. This multimodal device could identify important plaque features and their changes over multiple time points across the length of vessels containing atherosclerosis in high\u2010cholesterol diet\u2010fed apolipoprotein E knockout mice. It is found that OCT\u2010derived plaque lipid measures correlate closely with histologically assessed plaque lipid (Oil Red O) in co\u2010registered sections. Plaque ICG fluorescence also correlates with CD68+ macrophages, measured by immunofluorescence in co\u2010registered sections. This novel approach maximizes data acquisition of plaque evolution dynamics within individual vessels. It represents a paradigm shift over the current need to compare disparate atherosclerosis plaques histologically across multiple timepoints and in multiple animals.<\/jats:p>","DOI":"10.1002\/advs.77316","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:48:54Z","timestamp":1787147334000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serial Molecular\u2010Microstructural Multimodal Intravascular Imaging of Atherosclerotic Plaques in Mice"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8818-6070","authenticated-orcid":false,"given":"Jiawen","family":"Li","sequence":"first","affiliation":[{"name":"Discipline of Biomedical Engineering School of Electrical and Mechanical Engineering Adelaide University  Adelaide South Australia Australia"},{"name":"Institute for Photonics and Advanced Sensing Adelaide University  Adelaide South Australia Australia"},{"name":"Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics  Adelaide South Australia Australia"},{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1875-4882","authenticated-orcid":false,"given":"Joanne T. M.","family":"Tan","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Adelaide Medical School Adelaide University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4495-047X","authenticated-orcid":false,"given":"Lei","family":"Xiang","sequence":"additional","affiliation":[{"name":"Discipline of Biomedical Engineering School of Electrical and Mechanical Engineering Adelaide University  Adelaide South Australia Australia"},{"name":"Institute for Photonics and Advanced Sensing Adelaide University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3457-6513","authenticated-orcid":false,"given":"Jiandi","family":"Liu","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Department of Cardiology Second Affiliated Hospital Jiangxi Medical College Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3381-8779","authenticated-orcid":false,"given":"Ayla","family":"Hoogendoorn","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria A.","family":"Nankivell","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Adelaide Medical School Adelaide University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Sandeman","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Le","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Flinders Medical Centre  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rouyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Discipline of Biomedical Engineering School of Electrical and Mechanical Engineering Adelaide University  Adelaide South Australia Australia"},{"name":"Institute for Photonics and Advanced Sensing Adelaide University  Adelaide South Australia Australia"},{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6947-5061","authenticated-orcid":false,"given":"Robert A.","family":"McLaughlin","sequence":"additional","affiliation":[{"name":"Institute for Photonics and Advanced Sensing Adelaide University  Adelaide South Australia Australia"},{"name":"Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics  Adelaide South Australia Australia"},{"name":"School of Biomedicine Adelaide University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0222-5468","authenticated-orcid":false,"given":"Peter J.","family":"Psaltis","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Adelaide Medical School Adelaide University  Adelaide South Australia Australia"},{"name":"Department of Cardiology Royal Adelaide Hospital  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8336-6774","authenticated-orcid":false,"given":"Johan W.","family":"Verjans","sequence":"additional","affiliation":[{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Adelaide Medical School Adelaide University  Adelaide South Australia Australia"},{"name":"Department of Cardiology Royal Adelaide Hospital  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0682-8760","authenticated-orcid":false,"given":"Christina A.","family":"Bursill","sequence":"additional","affiliation":[{"name":"Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics  Adelaide South Australia Australia"},{"name":"Vascular Research Centre Lifelong Health Theme South Australian Health and Medical Research Institute (SAHMRI)  Adelaide South Australia Australia"},{"name":"Adelaide Medical School Adelaide University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"9349","article-title":"Global Epidemiology of Ischemic Heart Disease: Results from the Global Burden of Disease Study","volume":"12","author":"Khan M. 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W.","year":"2018","journal-title":"Scientific Reports"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2015.11.020"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2016.01.034"},{"key":"e_1_2_12_16_1","first-page":"200","article-title":"Apolipoprotein A\u2010I Reduces In\u2010Stent Restenosis and Platelet Activation and Alters Neointimal Cellular Phenotype","volume":"3","author":"Vanags L. Z.","year":"2018","journal-title":"JACC: Basic to Translation Science"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202107032"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41377\u2010020\u201000365\u2010w"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.09.079"},{"key":"e_1_2_12_20_1","first-page":"560","article-title":"Fully Integrated High\u2010Speed Intravascular Optical Coherence Tomography\/Near\u2010Infrared Fluorescence Structural\/Molecular Imaging In Vivo Using a Clinically Available Near\u2010Infrared Fluorescence\u2013Emitting Indocyanine Green to Detect Inflamed Lipid\u2010Rich Atheromata in Coronary\u2010Sized Vessels","volume":"7","author":"Lee S.","year":"2014","journal-title":"Circulation: Cardiovascular Interventions"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv726"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44325\u2010024\u201000016\u20108"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.2927464"},{"key":"e_1_2_12_24_1","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1109\/TMI.2005.854297","article-title":"Localized Measurement of Optical Attenuation Coefficients of Atherosclerotic Plaque Constituents By Quantitative Optical Coherence Tomography","volume":"24","author":"Meer F. 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