{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:46:01Z","timestamp":1787323561613,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Catheter\u2010related bloodstream infection (CRBSI) is an important clinical problem that causes significant mortality and excess economic cost. Recent epidemiological trends indicate a shift in CRBSI pathogens from Gram\u2010positive to Gram\u2010negative bacilli, among which\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    , and\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    predominate due to their strong antibiotic resistance and biofilm\u2010forming capabilities. Recently, bacteriophage\u2010encoded depolymerases have emerged as promising antivirulence agents that resensitize bacteria to host immunity by degrading capsular polysaccharides (CPS) and exopolysaccharides (EPS). In this study, we employed a mussel\u2010inspired polydopamine (PDA) coating technique to immobilize an\n                    <jats:italic>A. baumannii\u2010specific<\/jats:italic>\n                    depolymerase, DPO71, onto diverse organic, inorganic, and metal substrates, with a maximum surface coverage of\u200b \u223c0.2\u00a0\u00b5g\/cm\n                    <jats:sup>2<\/jats:sup>\n                    . In the presence of human serum, DPO71\u2010coated surfaces demonstrated potent antibacterial and antibiofilm activities. The coating significantly reduced bacterial burden in an epithelial cell\u2010bacteria co\u2010culture system and in ex vivo blood infection models. Moreover, DPO71 coatings demonstrated good robustness, stability, and minimal toxicity toward epithelial cells and\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    larvae\u200b. Importantly, in a mouse subcutaneous implantation model, the DPO71 coating significantly reduced bacterial load in vivo. Overall, modifying catheter surfaces with depolymerases via a simple PDA coating technique demonstrates great potential in mitigating bacteria\u2010associated CRBSI.\n                  <\/jats:p>","DOI":"10.1002\/advs.77319","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:09:28Z","timestamp":1787321368000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One\u2010Step Immobilized Phage Depolymerase Coatings for Catheter\u2010Related Bloodstream Infections"],"prefix":"10.1002","author":[{"given":"Lingkai","family":"Dong","sequence":"first","affiliation":[{"name":"School of Pharmacy The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingjin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Smart Materials and Future Energy Department of Chemistry Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Hu","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences Shanghai Key Laboratory of Medical Epigenetics Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Smart Materials and Future Energy Department of Chemistry Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry The Chinese University of Hong Kong, Shatin  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmacy The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6976-6157","authenticated-orcid":false,"given":"Zhong","family":"Zuo","sequence":"additional","affiliation":[{"name":"School of Pharmacy The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5615-6627","authenticated-orcid":false,"given":"Ronald Man Yeung","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics &amp; Traumatology The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1995-2274","authenticated-orcid":false,"given":"Tiancong","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Smart Materials and Future Energy Department of Chemistry Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0194-7058","authenticated-orcid":false,"given":"Sharon Shui Yee","family":"Leung","sequence":"additional","affiliation":[{"name":"School of Pharmacy The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"e32","DOI":"10.1016\/j.ehmc.2013.08.002","article-title":"Catheter\u2010Related Bloodstream Infections Review","volume":"3","author":"Abebe A.","year":"2014","journal-title":"Hospital Medicine Clinics"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S409400"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469\u20100691.12565"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1129729821990222"},{"key":"e_1_2_9_6_1","unstructured":"WHO publishes list of bacteria for which new antibiotics are urgently needed accessed:2017 https:\/\/www.who.int\/news\/item\/27\u201002\u20102017\u2010who\u2010publishes\u2010list\u2010of\u2010bacteria\u2010for\u2010which\u2010new\u2010antibiotics\u2010are\u2010urgently\u2010needed."},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.12968\/bjon.2021.30.8.S4"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2022.01.025"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879\u2010025\u201011367\u20107"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology14020165"},{"issue":"41","key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"36269","DOI":"10.1021\/acsami.7b07053","article-title":"In Vivo Anti\u2010Biofilm and Anti\u2010Bacterial Non\u2010Leachable Coating Thermally Polymerized on Cylindrical Catheter","volume":"9","author":"Zhou C.","year":"2017","journal-title":"ACS Applied Materials & Interfaces"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b19335"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6TB03280J"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.3390\/microorganisms12091835","article-title":"Microeukaryotes Associated With Freshwater Mussels in Rivers of the Southeastern United States","volume":"12","author":"Halliday\u2010Isaac A. 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