{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:05:32Z","timestamp":1789362332724,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tendinopathy arises from maladaptive cellular responses, though the drivers remain unclear. Here we isolate and characterise an overlooked tendon cell population residing within interfascicular matrix (IFM), demonstrating its importance as a highly mechanosensitive cell in tendon. We describe the first successful isolation and long\u2010term culture of enriched\u00a0populations of primary IFM and fascicular matrix (FM) cells, enabling direct comparison of their phenotypes and mechanosensitivity. IFM cells exhibited a potent response to stiff substrates, displaying cytoskeletal remodelling, rapid drifting of tenogenic and ECM gene expression, and proliferative decline, while FM cells remained largely unaltered. Crucially, transferring IFM cells to compliant, IFM\u2010like substrates recovered their proliferative capacity, morphology, and partially restored their gene expression. This work defines IFM cells as a highly mechanosensitive tendon cell population. Importantly, it also enables the establishment of a defined culture framework that supports the maintenance of key IFM phenotypic characteristics in vitro and provides a new perspective on tendon cell heterogeneity by identifying distinct compartment\u2010specific responses to the mechanical microenvironment.<\/jats:p>","DOI":"10.1002\/advs.77391","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T04:55:16Z","timestamp":1789361716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unravelling the Distinct Phenotype and Mechanosensitive Properties of Different Tendon Cell Populations"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1491-268X","authenticated-orcid":false,"given":"S. E.","family":"Grossemy","sequence":"first","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2839-7620","authenticated-orcid":false,"given":"D. E.","family":"Zamboulis","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. S.","family":"Khatib","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Fazal","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. C.","family":"Gains","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Giannopoulos","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0300-8230","authenticated-orcid":false,"given":"T.","family":"Hopkins","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5102-8542","authenticated-orcid":false,"given":"C.","family":"Bevan","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3755-1597","authenticated-orcid":false,"given":"M. M.","family":"Knight","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9077-0732","authenticated-orcid":false,"given":"H. R. C.","family":"Screen","sequence":"additional","affiliation":[{"name":"Centre for Predictive In Vitro Models Queen Mary University of London  London UK"},{"name":"School of Engineering and Materials Science Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1136\/bjsports-2019-100885","article-title":"ICON 2019: International Scientific Tendinopathy Symposium Consensus: Clinical Terminology","volume":"54","author":"Scott A.","year":"2020","journal-title":"British Journal of Sports Medicine"},{"key":"e_1_2_9_3_1","first-page":"9","article-title":"Critical Review on the Socio\u2010Economic Impact of Tendinopathy","volume":"4","author":"Hopkins C.","year":"2016","journal-title":"Asia\u2010Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"844","DOI":"10.3390\/ijms21030844","article-title":"Spectrum of Tendon Pathologies: Triggers, Trails and End\u2010State","volume":"21","author":"Steinmann S.","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.actbio.2016.06.012","article-title":"Fascicles and the Interfascicular Matrix Show Adaptation for Fatigue Resistance in Energy Storing Tendons","volume":"42","author":"Thorpe C. 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