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However, how the extracellular matrix (ECM) regulates cellular plasticity remains less known. Cellular plasticity is essential for successful metastasis, as cells undergo epithelial\u2010to\u2010mesenchymal transition and adherent\u2010to\u2010suspension transition (AST). Studies have begun to use 3D photo\u2010crosslinkable hydrogels, but, unlike in vivo ECM, hydrogel stiffness is inextricably linked to porosity. In this study, we propose a fragmented gelatin methacryloyl (GelMA) scaffold that controls stiffness independently from porosity. When encapsulated as single cells, non\u2010metastatic breast cancer cells do not exhibit growth restriction, whilst pre\u2010engineered metastatic breast cancer cells show altered mechanosensitivity and enhanced migration (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). We next study the role of AST in cellular migration and observe similar velocity to invasive metastatic cells. Interestingly, non\u2010metastatic cells showed AST\u2010dependent migration within interstitial spaces, which was enhanced in the stiff scaffold (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). AST induction significantly increased Lamin A\/C (associated with providing nuclear stability for circulating tumor cells) and reduced nuclear yes\u2010associated protein (YAP) (necessary for cell detachment). Our data highlights the importance of incorporating micro\u2010scale porosity and presents a promising platform for the study of cellular growth and migration.\n                  <\/jats:p>","DOI":"10.1002\/advs.77478","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:34:11Z","timestamp":1788143651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Space Jam\u2010Ming: Generating Interstitial Space Using Fragmented Granular GelMA to Investigate Novel Paradigms of Cancer Metastasis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0411-2547","authenticated-orcid":false,"given":"Danielle","family":"Vahala","sequence":"first","affiliation":[{"name":"School of Human Sciences The University of Western Australia  Perth Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang 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