{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T05:18:39Z","timestamp":1788239919067,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The choroid plexus (ChP) secretes cerebrospinal fluid (CSF) and forms the blood\u2010CSF barrier (BCSFB), playing indispensable roles in brain development and homeostasis. However, tractable human ChP models remain limited, with current organoid protocols largely overlooking the stem cell niche as a determinant of lineage commitment. Here, we report that a small number of ChP organoids sporadically emerge during skin organoid induction, and switching human embryonic stem cells (hESCs) culture from E8 to mTeSR1 medium\u2014preconditioning cells toward a neural\u2010primed state\u2014substantially enhances ChP organoid induction efficiency from &lt;3% to \u223c68%. Mechanistically, mTeSR1 culture suppresses BMP and WNT activity while upregulating neuroepithelial programs, thereby enabling robust ChP differentiation upon BMP4 exposure. The resulting organoids recapitulate key structural and functional attributes of native ChP, including polarized epithelium and CSF\u2010like fluid (iCSF) secretion. Notably, iCSF\u2010derived exosomes closely mirror the protein composition of native CSF exosomes. Furthermore, expression of the early ChP markers OTX2 and MSX1 suggest progressive specification toward the ChP fate. These ChP organoids likely represent a mixed ventricular identity with predominant hindbrain characteristics and exhibit selective susceptibility to SARS\u2010CoV\u20102 infection, validating their physiological relevance. This niche\u2010driven priming strategy provides a scalable platform for modeling ChP development, barrier transport, and neurological disorders.<\/jats:p>","DOI":"10.1002\/advs.77512","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:55:26Z","timestamp":1788238526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Niche\u2010Mediated Neural Priming Enables Robust and Scalable Generation of Human Choroid Plexus Organoids"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4129-5787","authenticated-orcid":false,"given":"Zhongliang","family":"Chen","sequence":"first","affiliation":[{"name":"Center For Tissue Engineering and Stem Cell Research Translation Medicine Research Center Guizhou Biomanufacturing Laboratory Guizhou Medical University  Guiyang China"},{"name":"Department of Neurosurgery The Affiliated Hospital of Guizhou Medical University  Guiyang 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Education School of Public Health Guizhou Medical University  Guiyang China"},{"name":"Collaborative Innovation Center For Prevention and Control of Endemic and Ethnic Regional Diseases Co\u2010Constructed By the Province and Ministry Guizhou Medical University  Guiyang 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