{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:59:46Z","timestamp":1790787586030,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    5\u2010Methylcytosine (m\n                    <jats:sup>5<\/jats:sup>\n                    C) is a vital RNA modification that regulates mRNA stability, splicing, and nuclear export. As a key m\n                    <jats:sup>5<\/jats:sup>\n                    C reader, ALYREF promotes tumorigenesis across various cancers; however, its precise role in endometrial cancer (EC) remains unknown. ALYREF expression and clinical significance in EC were evaluated using bioinformatics and immunohistochemistry. In vitro and in vivo assays examined its functional roles. Integrated multi\u2010omics (RNA\u2010seq, RIP\u2010seq, and\n                    <jats:italic>m<\/jats:italic>\n                    <jats:sup>5<\/jats:sup>\n                    C\u2010BS\u2010seq) and rescue assays identified downstream targets. RNA stability, nuclear\u2010cytoplasmic fractionation, site\u2010directed mutagenesis, dual\u2010luciferase, immunofluorescence, and Western blotting were performed to elucidate the underlying mechanisms. ALYREF is significantly upregulated in EC and serves as an independent prognostic factor for poor overall survival. Functional studies demonstrated that ALYREF markedly promotes EC cell proliferation, migration, invasion, and tumor growth. Mechanistically,\n                    <jats:italic>XRCC6<\/jats:italic>\n                    was identified as a direct functional target. ALYREF enhances\n                    <jats:italic>XRCC6<\/jats:italic>\n                    mRNA stability in an\n                    <jats:italic>m<\/jats:italic>\n                    <jats:sup>5<\/jats:sup>\n                    C\u2010dependent manner, subsequently activating canonical Wnt\/\u03b2\u2010catenin signaling to drive EC progression. Our findings reveal that ALYREF functions as a potential oncogenic driver in EC. The ALYREF\u2013m\n                    <jats:sup>5<\/jats:sup>\n                    C\u2013XRCC6 axis accelerates EC progression via Wnt\/\u03b2\u2010catenin signaling, highlighting a promising therapeutic target.\n                  <\/jats:p>","DOI":"10.1002\/advs.77548","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:00:31Z","timestamp":1790532031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["m\n                    <sup>5<\/sup>\n                    C Reader ALYREF Promotes the Progression of Endometrial Cancer by Activating the XRCC6\u2010Mediated Wnt\/\u03b2\u2010catenin Signaling Pathway"],"prefix":"10.1002","author":[{"given":"Linlin","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology The Second Hospital of Jilin University  Changchun China"},{"name":"Department of Radiation Oncology The Affiliated Wuxi People's Hospital of Nanjing Medical University  Wuxi Jiangsu China"},{"name":"Wuxi Medical Center Nanjing Medical University  Wuxi Jiangsu China"},{"name":"Wuxi People's Hospital  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Department of Biology Southern University of Science and Technology  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Second Hospital of Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Second Hospital of Jilin University  Changchun 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