{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:04:20Z","timestamp":1789621460808,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["513752256"],"award-info":[{"award-number":["513752256"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Conventional magnetic resonance imaging (MRI) often detects liver tumors after morphologic and vascular changes become apparent, whereas biomechanical alterations may precede visible tumor formation. Here, we evaluate multifrequency magnetic resonance elastography (mMRE)\u2010derived shear wave speed (SWS) and phase angle (\u03d5) covering stiffness and viscosity as non\u2010invasive biomechanical imaging markers for liver cancer formation and progression in a translational rabbit model. VX2 liver tumor\u2010bearing New Zealand White rabbits underwent five longitudinal multiparametric MRI scans over 14\u00a0days, including mMRE at clinically relevant frequencies on a 3\u00a0Tesla scanner. SWS and \u03d5 maps were compared with apparent diffusion coefficient (ADC) maps, histopathology, and proteomics. Tumor SWS and \u03d5 increased 2\u20133 days after implantation and were significantly higher than liver at two weeks (SWS:\n                    <jats:italic>p<\/jats:italic>\n                    =\u00a0.002; \u03d5:\n                    <jats:italic>p<\/jats:italic>\n                    =\u00a0.008).\u00a0Tumor ADC decreased significantly only from days 11\u201313 onward (day 14:\n                    <jats:italic>p<\/jats:italic>\n                    =\u00a0.02). Histopathology and proteomics demonstrated tumoral enrichment of collagen, proteoglycan, and glycosaminoglycan pathways compared to liver, suggesting matrix remodeling as a key contributor to increased stiffness and viscosity measured by mMRE. The early elevation of SWS and \u03d5 preceding ADC changes highlights mMRE as a promising tool for detecting tumor\u2010associated biomechanical remodeling and enabling earlier liver cancer diagnosis and monitoring beyond conventional MRI.\n                  <\/jats:p>","DOI":"10.1002\/advs.77626","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:39:28Z","timestamp":1789619968000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal Multifrequency MR Elastography Reveals Biomechanical Signatures of Progression in a Translational Liver Tumor Model"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6010-8340","authenticated-orcid":false,"given":"Justus","family":"Ramtke","sequence":"first","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"Experimental and Clinical Research Center (ECRC) A Cooperation of Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5427-7564","authenticated-orcid":false,"given":"Friederike","family":"Hesse","sequence":"additional","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"Berlin Institute of Health at Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin 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(ECRC) A Cooperation of Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6224-2441","authenticated-orcid":false,"given":"Yubei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"Experimental and Clinical Research Center (ECRC) A Cooperation of Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"Experimental and Clinical Research Center (ECRC) A Cooperation of Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanglei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-2799","authenticated-orcid":false,"given":"Robin","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"Experimental and Clinical Research Center (ECRC) A Cooperation of Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1698-2204","authenticated-orcid":false,"given":"Selma","family":"Saclier","sequence":"additional","affiliation":[{"name":"Department of Radiology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"Experimental and Clinical Research Center (ECRC) A Cooperation of Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3040-2364","authenticated-orcid":false,"given":"Salma A. 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