{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:00:54Z","timestamp":1790989254269,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA0910700"],"award-info":[{"award-number":["2021YFA0910700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Peptide cancer vaccines often suffer from poor lymphatic drainage, limited antigen\u2010presenting cell uptake, and weak innate immune activation. To overcome these barriers, we engineered the Self\u2010Assembling Peptide\u2010Adjuvant Conjugate (SaPAC) platform, which covalently links neoantigen epitopes to the TLR7 agonist 1V209 through site\u2010selective lysine conjugation. Synthesized by Fmoc solid\u2010phase peptide synthesis, SaPAC conjugates self\u2010assemble into cationic nanoparticles with hydrodynamic diameters of approximately 100\u2013200\u00a0nm. Compared with unconjugated self\u2010assembling peptide nanoparticles mixed with soluble adjuvant, SaPAC nanoparticles enhanced lymphatic drainage and antigen persistence in draining lymph nodes. Mechanistically, SaPAC activated the TLR7\u2010MyD88 axis, promoted plasmacytoid dendritic cell and macrophage recruitment, drove APC maturation (CD80\n                    <jats:sup>+<\/jats:sup>\n                    CD86\n                    <jats:sup>+<\/jats:sup>\n                    ), and enhanced MHC\u2010I cross\u2010presentation to amplify antigen\u2010specific CD8+ T\u2010cell priming. Therapeutically, SaPAC monotherapy suppressed tumor growth in B16\u2010OVA melanoma and MB49 bladder carcinoma models by increasing intratumoral infiltration of activated CD8+ T cells and NK cells. In an orthotopic 4T1 triple\u2010negative breast cancer model, multivalent SaPAC vaccination synergized with anti\u2010PD\u20101 blockade and achieved durable tumor suppression comparable to the Poly(I:C)\u2010adjuvanted benchmark without detectable systemic toxicity. Collectively, SaPAC represents a chemically defined platform that bridges innate and adaptive anti\u2010tumor immunity.\n                  <\/jats:p>","DOI":"10.1002\/advs.77632","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:40:25Z","timestamp":1790988025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Self\u2010Assembling Peptide\u2010Adjuvant Conjugate (SaPAC) Platform for Precision Cancer Immunotherapy"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9932-208X","authenticated-orcid":false,"given":"Yang\u2010Fan","family":"Wu","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"},{"name":"Centre of Cancer Medicine School of Clinical Medicine The University of Hong Kong Pokfulam Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6144-7226","authenticated-orcid":false,"given":"Jing\u2010Chu","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7834-1476","authenticated-orcid":false,"given":"Ye\u2010Fan","family":"Hu","sequence":"additional","affiliation":[{"name":"BayVax Biotech Limited Hong Kong Science Park Pak Shek Kok New Territories Hong Kong People's Republic of China"},{"name":"Shenzhen BayVax Biotech Limited Shenzhen People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Rong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences Shenzhen People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6383-3240","authenticated-orcid":false,"given":"Ren\u2010Hao","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"},{"name":"Centre of Cancer Medicine School of Clinical Medicine The University of Hong Kong Pokfulam Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6101-3804","authenticated-orcid":false,"given":"Yuan","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9574-0151","authenticated-orcid":false,"given":"Lehan","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao\u2010Zhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences Shenzhen People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9717-5112","authenticated-orcid":false,"given":"Yicheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacy Li Ka Shing Faculty of Medicine, The University of Hong Kong Pokfulam Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4588-5346","authenticated-orcid":false,"given":"Shing\u2010Fung","family":"Chow","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacy Li Ka Shing Faculty of Medicine, The University of Hong Kong Pokfulam Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canhui","family":"Su","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Yau","sequence":"additional","affiliation":[{"name":"Centre of Cancer Medicine School of Clinical Medicine The University of Hong Kong Pokfulam Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1382-719X","authenticated-orcid":false,"given":"Clive Yik\u2010Sham","family":"Chung","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"},{"name":"Department of Pathology School of Clinical Medicine Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7531-2816","authenticated-orcid":false,"given":"Jian\u2010Dong","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and School of Biomedical Engineering Li Ka Shing Faculty of Medicine The University of Hong Kong Pokfulam Hong Kong SAR People's Republic of China"},{"name":"Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences Shenzhen People's Republic of China"},{"name":"Centre for Virology, Vaccinology and Therapeutics Hong Kong Science and Technology Park Hong Kong Special Administrative Region People's Republic of China"},{"name":"Shenzhen Key Laboratory for Cancer Metastasis and Personalized Therapy Department of Clinical Oncology The University of Hong Kong\u2010Shenzhen Hospital Shenzhen Guangdong Province People's Republic of China"},{"name":"Materials Innovation Institute for Life Sciences and Energy (MILES) HKU\u2010SIRI Shenzhen People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1210044","article-title":"Peptide\u2010Based Vaccine for Cancer Therapies","volume":"14","author":"Buonaguro L.","year":"2023","journal-title":"Front Immunology"},{"issue":"10","key":"e_1_2_13_3_1","doi-asserted-by":"crossref","first-page":"2700","DOI":"10.1016\/j.bmc.2017.06.052","article-title":"Therapeutic Peptides: Historical Perspectives, Current Development Trends, and Future Directions","volume":"26","author":"Lau J. L.","year":"2018","journal-title":"Bioorganic & Medicinal Chemistry"},{"issue":"13","key":"e_1_2_13_4_1","doi-asserted-by":"crossref","first-page":"6011","DOI":"10.7150\/thno.38742","article-title":"Personalized Neoantigen Vaccination with Synthetic Long Peptides: Recent Advances and Future Perspectives","volume":"10","author":"Chen X.","year":"2020","journal-title":"Theranostics"},{"key":"e_1_2_13_5_1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.bioactmat.2021.09.029","article-title":"Peptide\u2010Based Nanomaterials: Self\u2010Assembly, Properties and Applications","volume":"11","author":"Li T.","year":"2022","journal-title":"Bioactive Materials"},{"issue":"1","key":"e_1_2_13_6_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s00726-025-03454-5","article-title":"Therapeutic Peptides: Chemical Strategies Fortify Peptides for Enhanced Disease Treatment Efficacy","volume":"57","author":"Li Q.","year":"2025","journal-title":"Amino Acids"},{"issue":"11","key":"e_1_2_13_7_1","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1056\/NEJMoa2032183","article-title":"Once\u2010Weekly Semaglutide in Adults with Overweight or Obesity","volume":"384","author":"Wilding J. P. H.","year":"2021","journal-title":"New England Journal of Medicine"},{"issue":"7","key":"e_1_2_13_8_1","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1158\/2159-8290.CD-25-0300","article-title":"The Future of Personalized Cancer Vaccines","volume":"15","author":"Martini D. J.","year":"2025","journal-title":"Cancer Discovery"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010026\u201003018\u20102"},{"issue":"9","key":"e_1_2_13_10_1","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1016\/j.ccell.2022.08.003","article-title":"Personalized Neoantigen Vaccine NEO\u2010PV\u201001 with Chemotherapy and Anti\u2010PD\u20101 as First\u2010Line Treatment for Non\u2010Squamous Non\u2010Small Cell Lung Cancer","volume":"40","author":"Awad M. M.","year":"2022","journal-title":"Cancer Cell"},{"issue":"19","key":"e_1_2_13_11_1","doi-asserted-by":"crossref","first-page":"5125","DOI":"10.1016\/j.cell.2025.06.019","article-title":"A Multi\u2010Adjuvant Personal Neoantigen Vaccine Generates Potent Immunity in Melanoma","volume":"188","author":"Blass E.","year":"2025","journal-title":"Cell"},{"issue":"7738","key":"e_1_2_13_12_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1038\/s41586-018-0792-9","article-title":"Neoantigen Vaccine Generates Intratumoral T Cell Responses in Phase Ib Glioblastoma Trial","volume":"565","author":"Keskin D. B.","year":"2019","journal-title":"Nature"},{"issue":"2","key":"e_1_2_13_13_1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.cell.2020.08.053","article-title":"A Phase Ib Trial of Personalized Neoantigen Therapy plus Anti\u2010PD\u20101 in Patients with Advanced Melanoma, Non\u2010Small Cell Lung Cancer, or Bladder Cancer","volume":"183","author":"Ott P. A.","year":"2020","journal-title":"Cell"},{"issue":"5","key":"e_1_2_13_14_1","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1158\/2159-8290.CD-24-0934","article-title":"PGV001, a Multi\u2010Peptide Personalized Neoantigen Vaccine Platform: Phase I Study in Patients with Solid and Hematologic Malignancies in the Adjuvant Setting","volume":"15","author":"Saxena M.","year":"2025","journal-title":"Cancer Discovery"},{"issue":"4","key":"e_1_2_13_15_1","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1038\/s41591-024-02894-y","article-title":"Personalized Neoantigen Vaccine and Pembrolizumab in Advanced Hepatocellular Carcinoma: A Phase 1\/2 Trial","volume":"30","author":"Yarchoan M.","year":"2024","journal-title":"Nature Medicine"},{"key":"e_1_2_13_16_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2025.115675","article-title":"Challenges and Opportunities on Achieving an Adequate Delivery Efficiency and Immunogenicity with Peptide\u2010Based Anticancer Vaccines","volume":"225","author":"Wang Y.","year":"2025","journal-title":"Advanced Drug Delivery Reviews"},{"issue":"32","key":"e_1_2_13_17_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2204078119","article-title":"Cell\u2010Penetrating Peptides Enhance Peptide Vaccine Accumulation and Persistence in Lymph Nodes to Drive Immunogenicity","volume":"119","author":"Backlund C. M.","year":"2022","journal-title":"Proceedings of the National Academy of Sciences USA"},{"issue":"11","key":"e_1_2_13_18_1","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1038\/nbt.3371","article-title":"In Vivo Characterization of the Physicochemical Properties of Polymer\u2010Linked TLR Agonists That Enhance Vaccine Immunogenicity","volume":"33","author":"Lynn G. M.","year":"2015","journal-title":"Nature Biotechnology"},{"issue":"3","key":"e_1_2_13_19_1","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/s41587-019-0390-x","article-title":"Peptide\u2013TLR\u20107\/8a Conjugate Vaccines Chemically Programmed for Nanoparticle Self\u2010Assembly Enhance CD8 T\u2010Cell Immunity to Tumor Antigens","volume":"38","author":"Lynn G. M.","year":"2020","journal-title":"Nature Biotechnology"},{"issue":"9","key":"e_1_2_13_20_1","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1158\/2326-6066.CIR-17-0607","article-title":"Enhancement of Peptide Vaccine Immunogenicity by Increasing Lymphatic Drainage and Boosting Serum Stability","volume":"6","author":"Moynihan K. D.","year":"2018","journal-title":"Cancer Immunology Research"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1125253"},{"issue":"11","key":"e_1_2_13_22_1","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1038\/nrd4608","article-title":"From Sewer to Saviour\u2014Targeting the Lymphatic System to Promote Drug Exposure and Activity","volume":"14","author":"Trevaskis N. L.","year":"2015","journal-title":"Nature Reviews Drug Discovery"},{"issue":"8","key":"e_1_2_13_23_1","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1021\/acs.bioconjchem.5b00285","article-title":"Enhancement of the Immunostimulatory Activity of a TLR7 Ligand by Conjugation to Polysaccharides","volume":"26","author":"Shinchi H.","year":"2015","journal-title":"Bioconjugate Chemistry"},{"issue":"19","key":"e_1_2_13_24_1","doi-asserted-by":"crossref","first-page":"7960","DOI":"10.1021\/acs.nanolett.1c01968","article-title":"Lymph\u2010Node\u2010Targeted Cholesterolized TLR7 Agonist Liposomes Provoke a Safe and Durable Antitumor Response","volume":"21","author":"Wan D.","year":"2021","journal-title":"Nano Letters"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.CIR\u201013\u20100223"},{"issue":"6","key":"e_1_2_13_26_1","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/s41551-020-0563-4","article-title":"Pharmacokinetic Tuning of Protein\u2010Antigen Fusions Enhances the Immunogenicity of T\u2010Cell Vaccines","volume":"4","author":"Mehta N. K.","year":"2020","journal-title":"Nature Biomedical Engineering"},{"issue":"1","key":"e_1_2_13_27_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/s41541-025-01199-6","article-title":"Optimized Lentiviral Backbone Induces Robust and Diverse T Cell Immunity against Neoantigens to Counteract Tumor Heterogeneity","volume":"10","author":"Vesin B.","year":"2025","journal-title":"npj Vaccines"},{"issue":"3","key":"e_1_2_13_28_1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1046\/j.1523-1747.2000.00084.x","article-title":"Formation and Kinetics of MHC Class I\u2010Ovalbumin Peptide Complexes on Immature and Mature Murine Dendritic Cells","volume":"115","author":"Kukutsch N. A.","year":"2000","journal-title":"Journal of Investigative Dermatology"},{"issue":"1","key":"e_1_2_13_29_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/bip.21328","article-title":"Self\u2010Assembly of Peptide Amphiphiles: From Molecules to Nanostructures to Biomaterials","volume":"94","author":"Cui H.","year":"2010","journal-title":"Biopolymers"},{"issue":"12","key":"e_1_2_13_30_1","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1038\/s41565-020-00782-3","article-title":"Proton\u2010Driven Transformable Nanovaccine for Cancer Immunotherapy","volume":"15","author":"Gong N.","year":"2020","journal-title":"Nature Nanotechnology"},{"issue":"8","key":"e_1_2_13_31_1","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1039\/D2SC07047B","article-title":"Hidden Hydrophobicity Impacts Polymer Immunogenicity","volume":"14","author":"Yuan Z.","year":"2023","journal-title":"Chemical Science"},{"issue":"12","key":"e_1_2_13_32_1","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1038\/s41576-019-0166-7","article-title":"Next\u2010Generation Computational Tools for Interrogating Cancer Immunity","volume":"20","author":"Finotello F.","year":"2019","journal-title":"Nature Reviews Genetics"},{"key":"e_1_2_13_33_1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.pharmthera.2014.09.010","article-title":"Poly(I:C) as Cancer Vaccine Adjuvant: Knocking on the Door of Medical Breakthroughs","volume":"146","author":"Ammi R.","year":"2015","journal-title":"Pharmacology & Therapeutics"},{"issue":"1","key":"e_1_2_13_34_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1186\/s13046-021-02017-2","article-title":"A Systematic Review on Poly(I:C) and Poly\u2010ICLC in Glioblastoma: Adjuvants Coordinating the Unlocking of Immunotherapy","volume":"40","author":"De Waele J.","year":"2021","journal-title":"Journal of Experimental & Clinical Cancer Research"},{"issue":"1","key":"e_1_2_13_35_1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s40425-018-0493-9","article-title":"\u03b1\u2010PD\u20101 Therapy Elevates Treg\/Th Balance and Increases Tumor Cell pSmad3 That Are Both Targeted by \u03b1\u2010TGF\u03b2 Antibody to Promote Durable Rejection and Immunity in Squamous Cell Carcinomas","volume":"7","author":"Dodagatta\u2010Marri E.","year":"2019","journal-title":"Journal for ImmunoTherapy of Cancer"},{"issue":"2","key":"e_1_2_13_36_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41587-019-0322-9","article-title":"A Large Peptidome Dataset Improves HLA Class I Epitope Prediction across Most of the human Population","volume":"38","author":"Sarkizova S.","year":"2020","journal-title":"Nature Biotechnology"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006063\u2010y"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature23003"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(23)02268\u20107"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/142919"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa379"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"issue":"4","key":"e_1_2_13_43_1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon Provides Fast and Bias\u2010Aware Quantification of Transcript Expression","volume":"14","author":"Patro R.","year":"2017","journal-title":"Nature Methods"},{"issue":"12","key":"e_1_2_13_44_1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA\u2010Seq Data with DESeq2","volume":"15","author":"Love M. I.","year":"2014","journal-title":"Genome Biology"},{"issue":"3","key":"e_1_2_13_45_1","article-title":"clusterProfiler 4.0: A Universal Enrichment Tool for Interpreting Omics Data","volume":"2","author":"Wu T.","year":"2021","journal-title":"Innovation"},{"issue":"1","key":"e_1_2_13_46_1","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1186\/s13059-017-1349-1","article-title":"xCell: Digitally Portraying the Tissue Cellular Heterogeneity Landscape","volume":"18","author":"Aran D.","year":"2017","journal-title":"Genome Biology"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.77632","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.77632","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.77632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:40:30Z","timestamp":1790988030000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.77632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,2]]},"references-count":45,"alternative-id":["10.1002\/advs.77632"],"URL":"https:\/\/doi.org\/10.1002\/advs.77632","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,2]]},"assertion":[{"value":"2026-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e77632"}}