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However, a reliable and quantitative SERS analysis in vivo is a great challenge since SERS is affected by the unevenly distributed field enhancement in hot spots of nanoparticle substrate and unpredictable nanoparticle aggregation in the complex biological environment. Here, we present non\u2010invasively quantitative Raman imaging through stacking\u2010induced intermolecular charge transfer\u2010enhanced Raman scattering (SICTERS) without relying on substrate. Based on the Raman imaging with SICTERS micelles, a quantitative method is established to accurately calculate the concentration of micelles in draining lymph nodes (DLNs) following subcutaneous administration. A physiologically based pharmacokinetic model is generated to fit the concentration\u2010time curve of the SICTERS micelles in DLNs (R\n                    <jats:sup>2<\/jats:sup>\n                    &gt; 0.99), which calculates the pharmacokinetic parameters of the lymphatic transport of micelles with different sizes and surface charges. The optimized SICTERS micelles loading ovalbumin (OVA) as model antigen exhibits significant anti\u2010tumor effect in mice bearing B16\u2010OVA melanoma following vaccination. This study demonstrates a quantitative in vivo Raman imaging platform based on SICTERS for image\u2010based pharmacokinetic modeling of micellar transport in lymphatics.\n                  <\/jats:p>","DOI":"10.1002\/advs.77634","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:23:08Z","timestamp":1788931388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Non\u2010Invasively Quantitative Raman Imaging for Calculation of Lymphatic Transport Kinetics of Micelles in Mice"],"prefix":"10.1002","author":[{"given":"Yunlu","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Minhang Hospital Key Laboratory of Smart Drug Delivery Ministry of Education State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzheng","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Minhang Hospital Key Laboratory of Smart Drug Delivery Ministry of Education State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1228-4516","authenticated-orcid":false,"given":"Sheng","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Minhang Hospital Key Laboratory of Smart Drug Delivery Ministry of Education State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunhui","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Minhang Hospital Key Laboratory of Smart Drug Delivery Ministry of Education State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Minhang Hospital Key Laboratory of Smart Drug Delivery Ministry of Education State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3457-5772","authenticated-orcid":false,"given":"Zeyu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology Institute of Molecular Medicine Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Hangzhou Institute of Medicine (HIM) Chinese Academy of Sciences  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1333-0274","authenticated-orcid":false,"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Minhang Hospital Key Laboratory of Smart Drug Delivery Ministry of Education State Key Laboratory of Molecular Engineering of Polymers Fudan University  Shanghai China"},{"name":"Quzhou Fudan Institute  Quzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100538\u20100"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c02545"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010021\u201001041\u2010z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c05359"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c06275"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr300120g"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"4946","DOI":"10.1021\/acs.chemrev.7b00668","article-title":"Surface\u2010Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges","volume":"118","author":"Zong C.","year":"2018","journal-title":"Chemical Reviews"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201203415"},{"key":"e_1_2_10_10_1","first-page":"668","article-title":"The Optical Properties of Metal Nanoparticles:\u2009 The Influence of Size, Shape, and Dielectric Environment","volume":"107","author":"Kelly K. 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