{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:08:00Z","timestamp":1790784480412,"version":"4.1.0"},"reference-count":100,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF17SA0028392"],"award-info":[{"award-number":["NNF17SA0028392"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF21OC0065495"],"award-info":[{"award-number":["NNF21OC0065495"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000864","name":"Michael J. Fox Foundation for Parkinson's Research","doi-asserted-by":"publisher","award":["MJFF\u2010021293"],"award-info":[{"award-number":["MJFF\u2010021293"]}],"id":[{"id":"10.13039\/100000864","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000864","name":"Michael J. Fox Foundation for Parkinson's Research","doi-asserted-by":"publisher","award":["MJFF\u2010026617"],"award-info":[{"award-number":["MJFF\u2010026617"]}],"id":[{"id":"10.13039\/100000864","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004836","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","award":["DFF4285\u201000247B"],"award-info":[{"award-number":["DFF4285\u201000247B"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009584","name":"Toyota Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009584","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Aggregation of \u03b1\u2010synuclein (\u03b1Syn) is a hallmark of Parkinson's disease, yet its pathological roles remain poorly understood. Elucidating these roles requires detailed knowledge of the free energy landscape of \u03b1Syn self\u2010assembly, i.e. the states that can be populated and their mechanisms and rates of interconversion. Here, we quantitatively probe this landscape using systematic mutational analysis, focusing on electrostatic contributions. We designed and produced 41 \u03b1Syn variants containing one to six acetyllysine\u2010mimicking (K\u2010Q) mutations to dissect the effects on key assembly pathways, fibril stability, and polymorphism. We derived a quantitative framework for the analysis of mutational effects and used it to isolate general electrostatic from residue\u2010specific effects on\n                    <jats:italic>de novo<\/jats:italic>\n                    \u03b1Syn aggregation. We established fibril stability and relative growth rate analysis as indirect biophysical probes of fibril polymorphism and identified K43Q+K45Q and K80Q as mutations with the most significant effect onamyloid formation. Moreover, we demonstrate that a subset of variants, most noticeably K58Q+K60Q, show differential sensitivity toward disease\u2010derived fibril polymorphs in seed amplification assays. Clustering analysis reveals that the mutational effects map onto the structural context of the lysine residues in known \u03b1Syn fibril structures. Together, this work provides a scalable, quantitative framework for probing the complex \u03b1Syn assembly landscape using mutational analysis.\n                  <\/jats:p>","DOI":"10.1002\/advs.77643","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:00:31Z","timestamp":1790532031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Probing the Energy Landscape of \u03b1\u2010Synuclein Amyloid Fibril Formation by Systematic K\u2010to\u2010Q Mutagenesis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1170-165X","authenticated-orcid":false,"given":"Antonin","family":"Kunka","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azad","family":"Farzadfard","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6782-0594","authenticated-orcid":false,"given":"Jacob Aunstrup","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9120-2745","authenticated-orcid":false,"given":"Rasmus Krogh","family":"Norrild","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8842-6319","authenticated-orcid":false,"given":"Federica","family":"Saraceno","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2039-6732","authenticated-orcid":false,"given":"Celia","family":"Fricke","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2303-795X","authenticated-orcid":false,"given":"Hossein","family":"Mohammad\u2010Beigi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0724-7990","authenticated-orcid":false,"given":"Ahmed","family":"Sadek","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Design and Immunoengineering Institute of Bioengineering, School of Life Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0940-3098","authenticated-orcid":false,"given":"Jonas","family":"Folke","sequence":"additional","affiliation":[{"name":"Centre For Neuroscience and Stereology Department of Neurology Copenhagen University Hospital Bispebjerg and Frederiksberg Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7940-6246","authenticated-orcid":false,"given":"Susana","family":"Aznar","sequence":"additional","affiliation":[{"name":"Centre For Neuroscience and Stereology Department of Neurology Copenhagen University Hospital Bispebjerg and Frederiksberg Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1161-3622","authenticated-orcid":false,"given":"Alexander K.","family":"Buell","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000112832"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08\u201008\u201002804.1988"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2012.07.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.639414"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2016.06.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22\u201008\u201003090.2002"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.302794"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2018.00388"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","unstructured":"R. S.Jacob A.Dema H.Ch\u00e9rot et\u00a0al. \u201c\u03b1\u2010Synuclein Acts as a Cholesteryl\u2010ester Sensor on Lipid Droplets Regulating Organelle Size and Abundance \u201dpreprint bioRxiv June 20 2024 http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.06.19.599670.","DOI":"10.1101\/2024.06.19.599670"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010021\u201000501\u2010z"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.26.010302.081142"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41531\u2010025\u201000887\u20103"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021937\u20103"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14547"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33252-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aal1604"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11070931"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10020375"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010024\u201001043\u2010w"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2021.661117"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2023.1197853"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600933200"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24475"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddy326"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CP03432F"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00637"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16696"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.654333"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.1c01281"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/340122a0"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20102836(92)90561\u2010W"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359\u20100278(96)00056\u20109"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(02)00442\u20104"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201010789\u20106"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1604645113"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2023487118"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b07403"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010022\u201003059\u20108"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2022.55.7.073"},{"issue":"17","key":"e_1_2_9_41_1","first-page":"1743","article-title":"N\u2010Terminal Acetylation of \u03b1\u2010Synuclein Slows Down Its Aggregation Process and Alters the Morphology of the Resulting Aggregates","volume":"61","author":"Bell R.","journal-title":"Biochemistry 2022"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201046898\u20101"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3784\u201011.2012"},{"key":"e_1_2_9_44_1","article-title":"Investigation of All Disease\u2010Relevant Lysine Acetylation Sites in \u03b1\u2010Synuclein Enabled by Non\u2010canonical Amino Acid Mutagenesis","volume":"14","author":"Shimogawa M.","journal-title":"eLife"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010020\u20100480\u20106"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adh3457"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.073181508"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008919200"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8SC01109E"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201058899\u20109"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2315006121"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2012171118"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.06.035"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594\u2010020\u20100384\u2010x"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010024\u201001712\u20109"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102317\u20106"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010022\u201001239\u20107"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41531\u2010023\u201000604\u2010y"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315346111"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CP05563B"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.3608916"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.2c00379"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b08311"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja412105t"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2313465121"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178250"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.010"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201007004\u20105"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.48907"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3SC05371G"},{"key":"e_1_2_9_71_1","unstructured":"K. L.Callaghan \u201cThermodynamic Characterisation of Amyloid Fibrils \u201ddiss. thesis Apollo\u2014University of Cambridge Repository (2021) https:\/\/www.repository.cam.ac.uk\/handle\/1810\/334808."},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1039\/d5sc04927j"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.08.013"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.07.001"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594\u2010019\u20100322\u2010y"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1917914117"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05971-2"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.104566"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201026433\u20102"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422\u2010020\u20100299\u20104"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922741117"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010023\u201002550\u20108"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407280102"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2023.102579"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2017452118"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202419173"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010018\u20100508\u20102"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010021\u201001282\u20108"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2022.167859"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805750200"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201108040"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki387"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002650"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2020.106519"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201005319\u20103"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2000374"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-74627-3"},{"key":"e_1_2_9_98_1","unstructured":"L.Hong X.Liu T. C. T.Michaels andT. P. J.Knowles \u201cHamiltonian Dynamics of Saturated Elongation in Amyloid Fiber Formation \u201dpriprint bioRxiv November 12 2020 https:\/\/arxiv.org\/abs\/2011.06222."},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610659104"},{"key":"e_1_2_9_100_1","unstructured":"D.Phan N.Pradhan andM.Jankowiak \u201cComposable Effects for Flexible and Accelerated Probabilistic Programming in NumPyro \u201dpriprint bioRxiv December 24 2019 https:\/\/arxiv.org\/abs\/1912.11554."},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2025.07.005"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.77643","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.77643","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.77643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:32:48Z","timestamp":1790782368000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.77643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,27]]},"references-count":100,"alternative-id":["10.1002\/advs.77643"],"URL":"https:\/\/doi.org\/10.1002\/advs.77643","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,27]]},"assertion":[{"value":"2026-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e77643"}}