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The spectra exhibited common features associated with the RNA backbone, ribofuranose rings, and nitrogenous bases, reflecting the overall structural similarity of the viral RNAs. However, distinct differences were observed in spectral signatures related to \u03b2\u2010D\u2010ribofuranose vibrations, phosphate linkages, and RNA bases sensitive to secondary\u2010structure variations. Conventional principal component analysis provided only limited discrimination among variants and subvariants. In contrast, Raman barcodes captured subtle molecular\u2010scale structural differences and successfully distinguished all investigated strains according to their RNA secondary structures. The analysis identified vibrational markers associated with nucleotide chain length, backbone conformation, and ribofuranose dynamics. Several backbone\u2010 and base\u2010related signals exhibited hyperchromic behavior correlated with RNA secondary\u2010structure features, including hairpin content and stem length. These relationships may serve as indicators for the rapid detection of emerging variants. By encoding key spectral signatures, Raman barcodes provide a robust framework for accurate classification of SARS\u2010CoV\u20102 strains based on the molecular architecture of their RNAs.<\/jats:p>","DOI":"10.1002\/advs.77679","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:37:59Z","timestamp":1790825879000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Raman Analysis of RNA Nucleotide Strands From SARS\u2010CoV\u20102 Variants and Subvariants: A Step Forward in the Definition of \u201cRaman Genome\u201d"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9663-2429","authenticated-orcid":false,"given":"Giuseppe","family":"Pezzotti","sequence":"first","affiliation":[{"name":"Biomedical Engineering Center Kansai Medical University Hirakata Osaka Prefecture Japan"},{"name":"International Center For Biomedical Industrial Promotion Kansai Medical 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