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Herein, we report a magnetic portable electrochemical immunosensor utilizing a conductive, anti\u2010fouling hydrogel coating composed of sodium alginate, polyethylene glycol, and\n                    <jats:styled-content>g<\/jats:styled-content>\n                    raphene (SPG). Upon calcium\u2010ion triggering, the SPG solution forms a stable electrode hydrogel coating within 1\u00a0min, effectively resisting nonspecific adsorption while maintaining low\u2011impedance electron transfer. Guided by a 3D\u2010printed magnetic apparatus, the MpECis directly captures target\u2010bound magnetic complexes, enabling rapid enrichment and electrical readout within 25\u00a0min. By implementing time\u2011domain averaging over the enzymatic reaction plateau, the coefficient of variation of electrical signals generated by nanoenzyme catalysis was reduced to 3.4%, markedly improving measurement reproducibility. The clinical practicality of the SPG MpECis platform was validated through three independent cohorts, including detection of cardiac troponin I in 59 serum samples, quantification of extracellular vesicles in 47 plasma samples, and detection of C\u2010reactive protein in 17 fingertip blood samples. Compared with the reference method, the area under the curve of this method are 0.99, 0.93, and 1.00, respectively. This platform offers a practical route for stable electrochemical diagnostics in complex biological matrices and home\u2010based precision medicine.\n                  <\/jats:p>","DOI":"10.1002\/advs.77696","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:42:24Z","timestamp":1789623744000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid\u2010Forming Conductive Antifouling Hydrogel Coating Enables Magnetically Guided Electrochemical POCT for Direct at\u2011Home Blood Testing"],"prefix":"10.1002","author":[{"given":"Menghan","family":"Li","sequence":"first","affiliation":[{"name":"The Center for Clinical Molecular Medical Detection The First Affiliated Hospital of Chongqing Medical University  Chongqing People's Republic of China"},{"name":"College of Laboratory Medicine , Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education) Chongqing Medical 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