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While cooperative interactions mediated by metabolite exchange can stabilize microbial consortia, the specific interactions between these consortia and plants remain understudied, particularly regarding their application in plant disease control. Here, we isolate a natural microbial consortium comprising\n                    <jats:italic>Achromobacter<\/jats:italic>\n                    ,\n                    <jats:italic>Flavobacterium<\/jats:italic>\n                    , and\n                    <jats:italic>Bacillus<\/jats:italic>\n                    strains from cucumber rhizosphere soil, and identify the\n                    <jats:italic>Flavobacterium<\/jats:italic>\n                    strain as a methionine auxotroph. This study reveals the metabolic interactions among strains within a cross\u2010feeding\u2010based consortium. The\n                    <jats:italic>Achromobacter<\/jats:italic>\n                    and\n                    <jats:italic>Bacillus<\/jats:italic>\n                    primarily metabolize carbon and nitrogen sources, respectively, whereas the relevant genes in the\n                    <jats:italic>Flavobacterium<\/jats:italic>\n                    are significantly downregulated. The consortium develops significantly enhanced biofilms with a distinct stratified architecture, facilitating root endophytic colonization by\n                    <jats:italic>Achromobacter<\/jats:italic>\n                    and\n                    <jats:italic>Flavobacterium<\/jats:italic>\n                    . Endophytic\n                    <jats:italic>Flavobacterium<\/jats:italic>\n                    induces systemic resistance in plants, thereby enhancing resistance against phytopathogenic fungal infections. Our findings reveal a novel ecological strategy employed by auxotrophic\n                    <jats:italic>Flavobacterium<\/jats:italic>\n                    species for both microbial interactions and root endophytic colonization and provide a theoretical foundation for microbial consortium\u2010based microbial inoculants in plant disease management.\n                  <\/jats:p>","DOI":"10.1002\/advs.77725","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:31:31Z","timestamp":1790742691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced Endophytic Colonization of\n                    <i>Flavobacterium<\/i>\n                    Promotes Plant Resistance to Fusarium Wilt"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5675-8924","authenticated-orcid":false,"given":"Xianfeng","family":"Ye","sequence":"first","affiliation":[{"name":"Key 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Agriculture and Rural Affairs Nanjing Agricultural University  Nanjing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs Nanjing Agricultural University  Nanjing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs Nanjing Agricultural University  Nanjing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs Nanjing Agricultural University  Nanjing People's 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