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This study utilizes a surface display strategy to construct an adhesion\u2010enhanced\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    Nissle 1917 chassis. By presenting a truncated SpaC adhesin derived from\n                    <jats:italic>Lactobacillus rhamnosus<\/jats:italic>\n                    GG via the ice nucleation protein system, the engineered strain achieves enhanced mucosal anchoring and prolonged intestinal retention. To achieve an autonomous response, the probiotic platform integrates a calprotectin\u2010responsive\n                    <jats:italic>ykgMO<\/jats:italic>\n                    promoter to drive a CAT\u2010SOD\u2010GPx tripartite fusion system (TFS) and secretion of human trefoil factor 3 (TFF3). In the TFS, CAT and SOD mediate ROS scavenging, whereas GPx is proposed to serve as a non\u2010catalytic structural\u2010support component associated with improved folding and soluble expression. Combined with pH\u2010responsive Eudragit L100\u201055 microencapsulation to ensure gastric survival, this programmable system effectively alleviates inflammation, restores epithelial barrier integrity, and reverses oxidative damage in both prophylactic and therapeutic murine colitis models. Furthermore, the platform remodels the gut microbiota by suppressing opportunistic pathobionts such as\n                    <jats:italic>Escherichia\u2010Shigella<\/jats:italic>\n                    while simultaneously promoting the resurgence of beneficial taxa, including\n                    <jats:italic>Lachnospiraceae<\/jats:italic>\n                    . Together, this biomarker\u2010responsive strategy offers a versatile and programmable framework for the precision management of IBD during acute flare\u2010ups.\n                  <\/jats:p>","DOI":"10.1002\/advs.77743","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:57:01Z","timestamp":1789131421000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adhesion\u2010Enhanced Engineered Probiotic for Prolonged Intestinal Retention and Calprotectin\u2010Responsive IBD Therapy"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3082-904X","authenticated-orcid":false,"given":"Yuxi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Food Science and Nutrition Zhejiang University  Hangzhou China"},{"name":"Future Food Laboratory Innovation Centre of Yangtze River Delta Zhejiang University  Jiaxing 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