{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:59:02Z","timestamp":1790791142257,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015614","name":"Corteva Agriscience","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015614","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Microspore\u2010derived embryogenesis (MDE) is essential for doubled haploid (DH) plant breeding, but conventional bulk culture systems are limited by poor scalability and difficulty for automation. Adapting droplet microfluidics for plant cell culture is promising but challenging due to the large size and fragility of plant cells involved in embryogenesis, such as maize microspores (60\u201385\u00a0\u00b5m) and their expanding embryo\u2010like structures (200\u2013500\u00a0\u00b5m). Here, we present a droplet microfluidic platform engineered for large plant cells, integrating a tubing\u2010based loading strategy that minimizes sedimentation losses, robust generation of stable 20 and 200\u00a0nL droplets optimized for large\u2010cell encapsulation, and scalable 3D reservoir incubation for long\u2010term culture. Optimized flow conditions and surface treatments enabled cell encapsulation with high fidelity, sustaining viability for over two weeks. Multi\u2010modal viability assessment using fluorescence spectroscopy and microscopy (bright\u2010field and fluorescence) confirmed that droplet culture preserves cell health comparably to bulk methods. The platform supports both single\u2010cell and few\u2010cell encapsulation modes, and increasing droplet volume and cell loading density further enhances embryogenesis rates and total embryo yield, respectively. Together, these advances establish a scalable, modular droplet microfluidic platform for high\u2010throughput embryogenesis analysis and embryo production, offering new opportunities in plant developmental biology, precision breeding, and doubled\u2010haploid technology.<\/jats:p>","DOI":"10.1002\/advs.77936","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:00:31Z","timestamp":1790532031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Scalable Droplet Microfluidic Platform That Overcomes Large\u2010Cell Constraints for Long\u2010Term Plant Cell Culture and Embryogenesis Analysis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7045-7121","authenticated-orcid":false,"given":"Arman","family":"Mirmiran","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2080-3567","authenticated-orcid":false,"given":"Fangchi","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Datto","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5075-0748","authenticated-orcid":false,"given":"Tathansh","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1847-1778","authenticated-orcid":false,"given":"Elizabeth","family":"Chatt","sequence":"additional","affiliation":[{"name":"Corteva Agriscience  Johnston Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0688-1454","authenticated-orcid":false,"given":"Yashar","family":"Bashirzadeh","sequence":"additional","affiliation":[{"name":"Corteva Agriscience  Johnston Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Stessman","sequence":"additional","affiliation":[{"name":"Corteva Agriscience  Johnston Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9958-9239","authenticated-orcid":false,"given":"Lai","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuangwen","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hena","family":"Guo","sequence":"additional","affiliation":[{"name":"Corteva Agriscience  Johnston Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3540-9354","authenticated-orcid":false,"given":"Tza\u2010Huei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Johns Hopkins University  Baltimore USA"},{"name":"Department of Biomedical Engineering Johns Hopkins University  Baltimore USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top108437"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.07.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057234"},{"key":"e_1_2_9_5_1","first-page":"227","volume-title":"Progress in Botany Vol. 81","author":"Testillano P. 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