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Here, we identified four distinct polysaccharide fractions from\n                    <jats:italic>Ganoderma applanatum<\/jats:italic>\n                    (GAPs) and pinpointed GAP2 as the principal bioactive component that significantly ameliorates DSS\u2010induced colitis and intestinal barrier injury. Furthermore, experiments using germ\u2010free mice and fecal microbiota transplantation show that the barrier\u2010repairing efficacy of GAP2 depends on the gut microbiota and their metabolic outputs. This indicates that GAP2 acts as a microbiota\u2010directed modulator, exerting health benefits by enriching\n                    <jats:italic>Phocaeicola vulgatus<\/jats:italic>\n                    and tridecylic acid.\n                    <jats:italic>P. vulgatus<\/jats:italic>\n                    degrades GAP2 via GH16, facilitating fatty\u2010acid metabolism to generate tridecanoic acid (absent in the mutant), which may activate the Rho\/CDC42 pathway in intestinal epithelial cells to promote tight\u2010junction assembly, thereby restoring barrier integrity. Notably, a meta\u2010analysis of UC patient cohorts revealed that both the abundance of\n                    <jats:italic>P. vulgatus<\/jats:italic>\n                    and its capacity for tridecylic acid biosynthesis were significantly decreased. Our findings establish GAP2 as a potent postbiotic inducer that repairs the intestinal barrier via a potential\n                    <jats:italic>P. vulgatus<\/jats:italic>\n                    \u2010tridecylic acid\u2010Rho\/CDC42 axis. This work highlights the therapeutic potential of targeting this newly identified microbe\u2010metabolite\u2010signaling axis for the treatment of UC and related mucosal inflammatory disorders.\n                  <\/jats:p>","DOI":"10.1002\/advs.78105","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:58:27Z","timestamp":1791352707000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Postbiotic\u2010Inducing\n                    <i>Ganoderma applanatum<\/i>\n                    Polysaccharide Repairs the Intestinal Barrier via a\n                    <i>Phocaeicola vulgatus \u2010<\/i>\n                    Tridecylic Acid Axis"],"prefix":"10.1002","author":[{"given":"Chuan","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu People's Republic of China"},{"name":"School of Food Science 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