{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:52:34Z","timestamp":1791132754795,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Host mucus is a major nutrient reservoir for gut\n                    <jats:italic>Bacteroidota<\/jats:italic>\n                    , yet the gene\u2010level determinants of mucin utilization remain poorly defined. In this study, we used a genome\u2010scale CRISPR interference library in\n                    <jats:italic>Bacteroides thetaiotaomicron<\/jats:italic>\n                    to systematically map fitness contributions across the mucin\u2010utilization network, spanning surface accessibility, glycan degradation, and intracellular sugar assimilation. Our screen identified 1,256 genes with condition\u2010dependent fitness divergence, which revealed a layered organization of mucin\u2010dependent growth. A prominent mucin\u2010specific fitness signature was enrichment of strains with repressed capsular polysaccharide loci. Subsequent competition and deletion experiments validated that specific capsules impede growth on mucin, supporting a surface\u2010accessibility trade\u2010off model. By contrast, most mucin\u2010induced carbohydrate\u2010binding and glycoside hydrolase genes showed negligible fitness contributions, consistent with functional redundancy in extracellular glycan capture and processing. Conversely, downstream sugar catabolism showed pronounced condition\u2010specific constraints, with the galactose\u2010utilization gene BT_0623, causing measurable fitness defects upon repression in both mucin\u2010 and galactose\u2010containing media. Finally, comparison with murine colon CRISPRi data revealed broad concordance with the in vitro mucin fitness landscape, while also highlighting environment\u2010specific differences, in CPS\u2010 and polysaccharide degradation\u2010associated functions. Together, these results define mucin utilization as a layered fitness architecture governed by surface\u2010accessibility costs, redundant extracellular glycan processing, and downstream metabolic constraints.\n                  <\/jats:p>","DOI":"10.1002\/advs.78118","type":"journal-article","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:18:03Z","timestamp":1791130683000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determination of Gene\u2010Level Fitness Contributions Across the Mucin\u2010Utilization Network of\n                    <i>Bacteroides thetaiotaomicro<\/i>\n                    <i>n<\/i>"],"prefix":"10.1002","author":[{"given":"Kangsan","family":"Kim","sequence":"first","affiliation":[{"name":"Graduate School of Engineering Biology Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"KAIST Institute for the Bioinnovation Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7308-6970","authenticated-orcid":false,"given":"Jiwon","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering Biology Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"KAIST Institute for the Bioinnovation Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjeong","family":"Kang","sequence":"additional","affiliation":[{"name":"KAIST Institute for the Bioinnovation Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"Department of Biological Sciences Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5060-0344","authenticated-orcid":false,"given":"Sung Sun","family":"Yim","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering Biology Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"KAIST Institute for the Bioinnovation Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"Department of Biological Sciences Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1107-7380","authenticated-orcid":false,"given":"Donghui","family":"Choe","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Sungkyunkwan University  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4788-4184","authenticated-orcid":false,"given":"Byung\u2010Kwan","family":"Cho","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering Biology Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"KAIST Institute for the Bioinnovation Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"},{"name":"Department of Biological Sciences Korea Advanced Institute of Science and Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.26"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1306070110"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1109051"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004773"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2008.09.007"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8481"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2017.00111"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01137"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201010068\u20105"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113009"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac5992"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108789"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.10.043"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.007"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00095\u201020"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00860\u201016"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01441\u201014"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201058660\u20102"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.008094"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12228"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030287"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.228841"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12\u2010221416"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.17.9833"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080029"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf1166"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201004209\u20105"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.022"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00353-25"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201004899\u2010x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216244120"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.08.020"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010024\u201001642\u20109"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2026.2644983"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201032021\u20109"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjsbx.2022.100084"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709865200"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.131275"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzh094"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2632"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwaa097"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21725"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003967\u20105"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010023\u201001501\u20101"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010020\u20100746\u20105"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00729\u201020"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81212"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.08.003"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00228-23"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001221"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010017\u20100079\u20101"},{"key":"e_1_2_9_53_1","doi-asserted-by":"crossref","unstructured":"S. R.Schaus C.Jin G.Raba et\u00a0al. \u201cDegradation of MucinO\u2010Glycans by a Human Gut Symbiont Requires a Complex Enzyme Repertoire and Promotes Colonization \u201dpreprintbioRxivMarch 26 2026 https:\/\/doi.org\/10.64898\/2026.03.26.714468.","DOI":"10.64898\/2026.03.26.714468"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010018\u20100258\u20108"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12714"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1704367114"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00048.2013"},{"key":"e_1_2_9_58_1","doi-asserted-by":"crossref","unstructured":"G.Prezza R. T.Fansler T.Guest et\u00a0al. \u201cAn RNA Regulates Iron Homeostasis and Host Mucus Colonization inBacteroides thetaiotaomicron \u201dBioRxiv(2025): 2025.09.08.672848.","DOI":"10.1101\/2025.09.08.672848"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01762\u201019"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3047"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.03.007"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201013483\u2010w"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.78118","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:18:04Z","timestamp":1791130684000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.78118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,4]]},"references-count":63,"alternative-id":["10.1002\/advs.78118"],"URL":"https:\/\/doi.org\/10.1002\/advs.78118","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,4]]},"assertion":[{"value":"2026-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e78118"}}