{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:04:02Z","timestamp":1790784242985,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82430083"],"award-info":[{"award-number":["82430083"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sepsis arises from heterogeneous host\u2010cell states that influence bacterial clearance, yet single\u2010cell transcriptomic approaches do not directly connect microbial signals with host transcriptional programs. This study introduces single\u2010cell host\u2013bacterial 16S co\u2010sequencing (scH16S\u2010seq), which couples host transcriptomes with barcode\u2010resolved bacterial 16S\u2010derived UMI signals to resolve bacterial signal\u2010enriched host\u2010cell states. Applied to\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (KP) sepsis, scH16S\u2010seq mapped cell\u2010associated bacterial 16S\u2010derived signals to discrete\n                    <jats:italic>\n                      CD38\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:italic>\n                    myeloid states, including Acod1\n                    <jats:sup>+<\/jats:sup>\n                    monocyte\u2010derived macrophages,\n                    <jats:italic>Lyve1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    interstitial macrophages, and hypoxic neutrophils sharing inflammatory, hypoxic, and metabolically stressed programs. Increased myeloid CD38 expression is also observed across human sepsis cohorts. Mechanistically, KP induces CD38\u2010associated NAD\n                    <jats:sup>+<\/jats:sup>\n                    depletion, mitochondrial bioenergetic failure, reduced ATP, and impaired lysosomal acidification. CD38 inhibition, NAD\u2010related metabolic intervention, or\n                    <jats:italic>Cd38<\/jats:italic>\n                    deficiency restores NAD homeostasis and mitochondrial function, whereas blockade of mitochondrial ATP synthesis or V\u2010ATPase\u2010dependent acidification attenuates lysosomal and antibacterial rescue. Lyz2\u2010Cre\u2010mediated\n                    <jats:italic>Cd38<\/jats:italic>\n                    deletion improves bacterial control and sepsis outcomes in vivo. Together, scH16S\u2010seq resolves KP 16S signal\u2010enriched myeloid states and identifies a targetable CD38\u2013NAD\u2013mitochondrial\u2013lysosomal axis linking immunometabolic dysfunction to impaired antibacterial defense in sepsis.\n                  <\/jats:p>","DOI":"10.1002\/advs.78127","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:12:40Z","timestamp":1790781160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["scH16S\u2010Seq Maps\n                    <i>Klebsiella pneumoniae<\/i>\n                    \u2010Associated Myeloid States and Reveals a CD38\u2013NAD Immunometabolic Axis That Impairs Lysosomal Acidification in Sepsis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2453-6083","authenticated-orcid":false,"given":"Yangguang","family":"Li","sequence":"first","affiliation":[{"name":"Research Institute of General Surgery Jinling 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