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In this work, we studied the ability to guide muscle cell development using femtosecond\u2010laser\u2010induced topography on standard culture plates. This technique allows the creation of highly porous microstructured channels that exhibit strong hydrophobicity while offering mechanical cues for cell guidance. We characterized the resulting engineered surfaces by assessing their capacity to direct cellular organization. Myoblasts cultured on laser\u2010structured (LS) surfaces generated more mature myotubes exhibiting enhanced alignment and elongation compared with those cultured on standard plates. We applied the LS surfaces as a proof\u2010of\u2010concept approach to characterize myotube morphology in two common myopathies, Duchenne muscular dystrophy and facioscapulohumeral muscular dystrophy. The superior organization of the myotubes enabled a more accurate assessment at the single\u2010fiber level, revealing disease\u2010specific phenotypes. Here, we present the characterization and validation of an accessible, rapid, and reliable laser\u2010based method for generating micrometric topographies on standard culture plates tailored to skeletal muscle. The effectiveness and versatility of LS supports provide initial evidence for their use in disease modelling and support their future application in therapeutic screening and personalized medicine.<\/jats:p>","DOI":"10.1002\/advs.78129","type":"journal-article","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:06:16Z","timestamp":1791129976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrafast Micrometric Laser Structuring of Polymer Surfaces for Highly Organized Skeletal Muscle Tissue Modeling"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7269-2013","authenticated-orcid":false,"given":"Lucas","family":"Duvert","sequence":"first","affiliation":[{"name":"Marseille Medical Genetics, MMG Aix\u2010Marseille University INSERM  Marseille 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