{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:51:38Z","timestamp":1791352298997,"version":"4.3.1"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The blood\u2013brain\u2013tumor barrier (BBTB) penetration by anticancer drugs and their effects on glioblastoma (GBM) are assessed using 3D bioprinted\u2010spheroids. Comparative analysis reveals prominent cytoplasmic mixing between pericytes and the human GBM cell line U\u201087MG, expressions of tight junction proteins ZO\u20101, occludin, and claudin\u20105, which act as a barrier to the paracellular transport across BBTB, and the expression of ABC transporter such as P\u2010glycoprotein (P\u2010gp) in the vascular endothelial cell layer of BBTB spheroids. Anticancer drug\u2010induced U\u201087 MG cell death is quantified through in situ imaging of spheroids using a cancer cell death probe. Paclitaxel and cisplatin, known P\u2010gp substrates, do not demonstrate transcellular transport in BBTB spheroids, whereas temozolomide and a ruthenium (Ru) compound induce marked U\u201087 MG cell death. Relative BBTB permeability is calculated as a function of IC\n                    <jats:sub>50<\/jats:sub>\n                    and treatment concentration. The Ru compound, a partial P\u2010gp substrate, exhibits greater anticancer efficacy compared with temozolomide, which is not a P\u2010gp substrate, in BBTB spheroids. The Ru compound attenuates tight junction protein expression (occludin and ZO\u20101) in vascular endothelial cells through the released reactive oxygen species (ROS), increases ECM degradation via elevated matrix metalloproteinase\u20109 in vascular endothelial and astrocytes, and promotes expression of the vasoconstrictor endothelin\u20101.\n                  <\/jats:p>","DOI":"10.1002\/advs.78150","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:44:23Z","timestamp":1791351863000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["3D Bioprinted Blood\u2013Brain\u2013Tumor Barrier Spheroid\u2010Based In Situ Evaluation of Anticancer Compounds Against Glioblastoma: ROS\u2010Producing Ruthenium as a Potential Therapeutic Candidate"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5162-8030","authenticated-orcid":false,"given":"Hyunjung","family":"Lee","sequence":"first","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5694-6724","authenticated-orcid":false,"given":"Chan Ho","family":"Jeon","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0216-2572","authenticated-orcid":false,"given":"Doyun","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7797-3380","authenticated-orcid":false,"given":"Minjae","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3572-7705","authenticated-orcid":false,"given":"Minji","family":"Kwon","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9896-5892","authenticated-orcid":false,"given":"Joon Myong","family":"Song","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23309"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2020.188458"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/noajnl\/vdab100"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202003937"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-017-2626-2"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2003.11.021"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abi9119"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26845"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c19291"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/aba5f1"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202000540"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1903542116"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101402"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202511856"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2022.147937"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3407"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202106860"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15623"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0909-x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760902751902"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi020137x"},{"issue":"1","key":"e_1_2_11_23_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/4356386","article-title":"Oxidative Stress\u2010Mediated Blood\u2010Brain Barrier (BBB) Disruption in Neurological Diseases","volume":"2020","author":"Song K.","year":"2020","journal-title":"Oxidative medicine and cellular longevity"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c07785"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2020.100561"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2024.113751"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.07.030"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-1836"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201902971"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800076105"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715761003667554"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.2741\/1667"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.105"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2016.03.042"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201916400"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004317"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nou012"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1203\u20101041"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003827107"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01095"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2024.2361202"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3923"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2000.1178"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2023.1267785"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2014.04.021"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.001503"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2009.12.002"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.5.541"},{"key":"e_1_2_11_49_1","first-page":"397","volume-title":"Molecular and Cellular Biology of Neuroprotection in the CNS, (Ed.:^Eds.: C. Alzheimer)","author":"Pardridge W. M.","year":"2002"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010021\u201008972\u20105"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2012.06.009"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16655551"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3546"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-014-1442-1"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25193"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78150","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.78150","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:44:25Z","timestamp":1791351865000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.78150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":54,"alternative-id":["10.1002\/advs.78150"],"URL":"https:\/\/doi.org\/10.1002\/advs.78150","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"2026-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e78150"}}