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Here, we developed a bioadaptive modular implant integrating porous titanium (Ti) alloy for structural reinforcement with spatially localized \u03b2\u2010tricalcium phosphate (\u03b2\u2010TCP) modules for bioactive regulation. Using a gelatin methacryloyl (GelMA)\u2010based diffusion\u2010confined model, we identified spatial differences in \u03b2\u2010TCP\u2010associated calcium distribution and corresponding position\u2010dependent changes in hBMSC proliferation, adhesion, osteogenic differentiation, and Wnt\/\u03b2\u2010catenin\u2010related signaling. The proximal region showing the strongest biological responses was used as a model\u2010derived spatial reference for organizing \u03b2\u2010TCP\u2010containing modules. In a rabbit femoral segmental defect model, the Ti alloy\/\u03b2\u2010TCP assembly implant showed greater bone formation and ingrowth than porous Ti alloy controls. Histology further revealed that newly formed bone was preferentially associated with \u03b2\u2010TCP\u2010containing regions and extended into adjacent porous Ti structures. These findings demonstrate a proof\u2010of\u2010concept for translating experimentally characterized spatial biological responses into the spatial organization of structural and bioactive implant components.<\/jats:p>","DOI":"10.1002\/advs.78161","type":"journal-article","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:26:11Z","timestamp":1791131171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4856-7148","authenticated-orcid":false,"given":"Heng","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology  Guangzhou People's Republic of China"},{"name":"National Engineering Research Center For Tissue Restoration and 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9bm00561g"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.07.017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.08.039"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.10045"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2021.01.043"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2011.06.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.J.01513"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/coatings13061091"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s10534-006-9029-8","article-title":"Signal Transduction in Monocytes: The Role of 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