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Highly biomimetic organoids and organs\u2010on\u2010chips improve relevance but at a cost that is incompatible with early screening campaigns. Photopatterned hydrogel scaffolds are potentially scalable, but current approaches lack plate\u2010scale reproducibility and throughput. To address this gap, we present chemically\u2010mediated Light\u2010Intensity Gradients for structured hydrogel functionalization of ultra\u2010High\u2010Throughput Screening formats (LigHTS): an all\u2010optical, in\u2010well method for parallel photofabrication of biomimetic hydrogels in standard 384\u2010 and 1536\u2010well plates. Adding ultraviolet\u2010light absorber tartrazine to photo\u2010curable solutions enables hydrogel thickness control by tuning illumination dose without focusing optics. Entire plates are functionalized in parallel with soft (\u223c1\u201310 kPa), thin (\u223c10\u2013200 \u00b5m) hydrogels. 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