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Here, we established a modular platform capable of efficiently inducing and long\u2010term expanding porcine germ cell\u2010like cells directly from pluripotent stem cells. We first developed a one\u2010step method to induce pro\u2010spermatogonia\u2010like cells (activating SOX17) and oogonia\u2010like cells (activating SOX17\/TFAP2C) from porcine pre\u2010gastrulation epiblast stem cells (pgEpiSCs) harboring a NANOS3\u2010tdTomato (NT) reporter, via activation of exogenous transcription factors. Single\u2010cell transcriptome profiling revealed a differentiation trajectory from pgEpiSCs through primordial germ cell\u2010like cells (PGCLCs) to pro\u2010spermatogonia\/oogonia\u2010like cells, with concomitant differentiation of gonadal somatic cells. Functional analyses demonstrate that EGF activates core PGC network and is essential for porcine PGCLCs survival in a xenogeneic reconstituted ovary, BMP4 drives NANOS3 and DDX4 expression, and IWR1 enhances germ cell differentiation by suppressing mesodermal programs. Finally, to address the challenge of cell quantity, we established a long\u2010term expansion module based on FGF2, IWR1, and EGF that sustains NT\u2010positive cells for up to 120 days while preserving their early PGC and pro\u2010spermatogonia\u2010like molecular characteristics. Our study provides new insights into porcine gametogenesis and reproductive development across species.<\/jats:p>","DOI":"10.1002\/advs.78213","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:58:06Z","timestamp":1791777486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One\u2010Step Induction of Oogonia\u2010Like and Pro\u2010Spermatogonia\u2010Like Cells From Porcine Pluripotent Stem Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0497-721X","authenticated-orcid":false,"given":"Pengcheng","family":"He","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding Frontiers Science Center for Molecular Design Breeding (MOE) College of Biological Sciences China Agricultural University  Beijing 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