{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:50:52Z","timestamp":1792036252169,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 22322405"],"award-info":[{"award-number":["NSFC 22322405"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274055"],"award-info":[{"award-number":["22274055"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22674073"],"award-info":[{"award-number":["22674073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Histamine (HA) is a critical neurotransmitter, yet its intracellular dysregulation remains difficult to monitor due to the slow kinetics and environmental sensitivity of traditional fluorescent probes. Herein, we report a supramolecular ratiometric probe engineered via the host\u2013guest self\u2010assembly of cationic fluorophores with cucurbit[7]uril (CB[7]). By precisely modulating the cationic locus within the guests, we achieved a 2:1 (host:guest) stoichiometric complex (\n                    <jats:bold>\n                      CB[7]\n                      <jats:sub>2<\/jats:sub>\n                      \u2283GA\u20101\n                    <\/jats:bold>\n                    ) that displays a distinct 60\u00a0nm bathochromic shift. Functioning via a competitive displacement mechanism, this probe demonstrates exceptional selectivity and an ultrafast response time of 0.96 s (\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>obs<\/jats:sub>\n                    = 3.56 s\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    ). We successfully utilized this system to visualize real\u2010time HA fluctuations in living mast cells with sub\u2010second resolution. Notably, our imaging revealed a quantitative correlation between nigericin\u2010induced cytoplasmic HA elevation and mast cell rupture. These findings validate the probe's efficacy in tracking cellular inflammatory responses and highlight its potential as an early\u2010warning indicator for drug\u2010induced cytotoxicity. Consequently, this work offers a robust tool for dissecting HA\u2010related pathophysiology and establishes a generalizable design paradigm for the rapid, ratiometric sensing of biologically relevant analytes.\n                  <\/jats:p>","DOI":"10.1002\/advs.78254","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:27:36Z","timestamp":1792034856000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sub\u2010Second Ratiometric Fluorescence Imaging of Histamine Dynamics in Living Cells via a Supramolecular Probe"],"prefix":"10.1002","author":[{"given":"Chenming","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Chemistry School of Chemistry and Molecular Engineering East China Normal University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Chemistry and Molecular Engineering East China Normal University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Fei","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Chemistry and Molecular Engineering East China Normal University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Chemistry and Molecular Engineering East China Normal University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1527-0559","authenticated-orcid":false,"given":"Qi\u2010Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Chemistry and Molecular Engineering East China Normal University  Shanghai P. R. China"},{"name":"Institute of Magnetic Resonance and Molecular Imaging in Medicine East China Normal University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006681\u20106"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113073"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201050042\u20104"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.033"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15692"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2207003120"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16182"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201044477\u20104"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1920122117"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01980882"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00185E"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010023\u201001350\u20101"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41570\u2010026\u201000822\u2010x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c06682"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2026.217672"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5CS01304F"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5SC03880D"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201057100\u20105"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c09607"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c08271"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/d6sc05216a"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.02.024"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.6023\/a25050200"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2025.216785"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5CS01495F"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202523648"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3SC00355H"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chempr.2022.03.012"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c09239"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/10610278.2025.2584997"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios12030161"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04758"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c16437"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202104790"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201036869\u20103"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5c03235"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201405025"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.5c00774"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b04776"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c22172"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.4c00721"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c02032"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c00415"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.1c02684"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2023.07.012"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703490"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.206"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6c02241"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.1c01046"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927611012104"}],"container-title":["Advanced 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