{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:48:38Z","timestamp":1792043318114,"version":"4.4.0"},"reference-count":69,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372971"],"award-info":[{"award-number":["32372971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260817"],"award-info":[{"award-number":["32260817"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260026"],"award-info":[{"award-number":["32260026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Intestinal glucagon\u2010like peptide\u20101 (GLP\u20101) is secreted by intestinal L cells in a glucose\u2010dependent manner. The localization and surface abundance of glucose\u2010sensing proteins on the cell membrane affect the response to glucose, but the regulatory mechanism remains poorly understood. Here, we show that S\u2010palmitoylation is required for stable plasma membrane enrichment of SGLT1 and for SGLT1\u2010mediated GLP\u20101 secretion. Notably, myostatin (MSTN) was identified as a key upstream regulator of this modification. Intestinal MSTN deficiency markedly enhanced SGLT1 palmitoylation, increased its plasma membrane abundance, and thereby promoted glucose\u2010stimulated GLP\u20101 secretion. Importantly, simvastatin induced intestinal MSTN upregulation and impaired glucose homeostasis, whereas MSTN deficiency largely alleviated these effects. Mechanistically, MSTN regulated the expression of the palmitoyl acyltransferase ZDHHC20, which catalyzed SGLT1 S\u2010palmitoylation at Cys301. Blocking SGLT1 palmitoylation attenuated SGLT1 membrane enrichment and markedly suppressed the induction of GLP\u20101 secretion caused by MSTN deficiency. Collectively, our research results have revealed a new mechanism by which MSTN regulates intestinal endocrine function and S\u2010palmitoylation modification promotes GLP\u20101 secretion, providing potential targets for regulating endogenous GLP\u20101 secretion in the intestine and treating metabolic diseases related to glucose imbalance.<\/jats:p>","DOI":"10.1002\/advs.78302","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:26:33Z","timestamp":1792041993000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loss of Intestinal MSTN Promotes GLP\u20101 Secretion Through SGLT1 Palmitoylation"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3335-510X","authenticated-orcid":false,"given":"Sheng\u2010Zhong","family":"Han","sequence":"first","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang\u2010Yan","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5743-7865","authenticated-orcid":false,"given":"Zhaobo","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Medicine Beth Israel Deaconess Medical Center and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8411-343X","authenticated-orcid":false,"given":"Biaohu","family":"Quan","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"},{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqiu","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Emergency Guangzhou Institute of Emergency Medicine the Second Affiliated Hospital Guangzhou Medical University  Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouyan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitong","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meifu","family":"Xuan","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"},{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyufan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rihuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6882-8763","authenticated-orcid":false,"given":"Xi\u2010Jun","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"},{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7195-8078","authenticated-orcid":false,"given":"Lin\u2010Hu","family":"Quan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain College of Pharmacy Ministry of Education Yanbian University  Yanji People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0755-8069","authenticated-orcid":false,"given":"Jin\u2010Dan","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Animal Science Yanbian University  Yanji Jilin People's Republic of China"},{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University  Yanji Jilin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms27020967"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1181913"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81858"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010023\u201000860\u20108"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj\u20102022\u2010071727"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883\u2010024\u201001266\u20108"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565\u2010020\u201009558\u2010w"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2022.106237"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2025.120772"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2500106"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12050420"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2016.1168808"},{"issue":"11","key":"e_1_2_11_14_1","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1016\/j.bbamem.2013.06.007","article-title":"Up\u2010Regulation of Na+\u2010Coupled Glucose Transporter SGLT1 by Caveolin\u20101","volume":"1828","author":"Elvira B.","year":"2013","journal-title":"Biochimica et Biophysica Acta (BBA)\u2014Biomembranes"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12096"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.07.009"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2014.02.001"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.210390"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81858"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574\u2010023\u201000817\u20104"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2020.101014"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecco\u2010jcc\/jjaf167"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c03818"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010cellbio\u2010100814\u2010125218"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2008\u20100003"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.2337\/db14\u20100807"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.2337\/db17\u20100058"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201061875\u2010y"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2006.04.015"},{"issue":"6","key":"e_1_2_11_30_1","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/s41580-024-00700-8","article-title":"Mechanisms and Functions of Protein S\u2010Acylation","volume":"25","author":"Mesquita F. S.","year":"2024","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010025\u201001738\u20107"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.05.010"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2215590120"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.137992"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1400128111"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20108587(24)00040\u20108"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201058668\u20102"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13061"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2021.155120"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574\u2010024\u201000979\u20109"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255\u2010024\u201001113\u20109"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI148372"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI178303"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/1753\u20100407.12161"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.067207"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11\u20101029"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13255"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201051240\u2010w"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255\u2010025\u201001309\u20107"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021235\u2010y"},{"issue":"4","key":"e_1_2_11_51_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.106057","article-title":"Oral Metformin Transiently Lowers Post\u2010Prandial Glucose Response by Reducing the Apical Expression of Sodium\u2010Glucose Co\u2010Transporter 1 in Enterocytes","volume":"26","author":"Zubiaga L.","year":"2023","journal-title":"iScience"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2017.09.019"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE\u201014\u20100161"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010024\u201006293\u20103"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083360"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.116.104521"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1122895"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2017.1409191"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658\u2010025\u201000776\u2010w"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20160309"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108406"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209080"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2014.03.002"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010015\u20103528\u20105"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2023.12.027"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC\u201025\u20100721"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07935"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.110179"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16831"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201056344\u20105"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78302","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.78302","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:26:35Z","timestamp":1792041995000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.78302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":69,"alternative-id":["10.1002\/advs.78302"],"URL":"https:\/\/doi.org\/10.1002\/advs.78302","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"2026-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e78302"}}