{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:37:49Z","timestamp":1792730269927,"version":"4.5.0"},"reference-count":64,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82571063"],"award-info":[{"award-number":["82571063"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82530030"],"award-info":[{"award-number":["82530030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270954"],"award-info":[{"award-number":["82270954"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82501102"],"award-info":[{"award-number":["82501102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7242174"],"award-info":[{"award-number":["7242174"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025M771755"],"award-info":[{"award-number":["2025M771755"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025M781682"],"award-info":[{"award-number":["2025M781682"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The periosteum plays a central role in skeletal regeneration by serving as a reservoir of skeletal stem cells and a dynamic regenerative niche. However, in vitro models that faithfully preserve its cellular complexity, long\u2010term stability, and functional capacity remain limited, restricting mechanistic studies and translational applications. Here, we establish a long\u2010term expandable periosteal organoid system that can be stably propagated for over 20 passages while preserving cellular heterogeneity, multilineage differentiation potential, and genomic integrity. Single\u2010cell transcriptomic analysis reveals sustained maintenance of core periosteal lineages and regenerative signaling programs over extended culture. Functionally, these organoids reconstruct a niche\u2010like microenvironment that supports hematopoietic stem cell (HSC) maintenance, preserves stem cell fitness, and attenuates aging\u2010associated functional decline under prolonged ex vivo conditions. Upon transplantation, periosteal organoids robustly engraft and actively participate in tissue repair, promoting vascularized bone formation and reconstruction of marrow\u2010like structures across multiple defect models. Mechanistically, we identify ZFP575 as a 3D niche\u2010dependent regulator required for maintaining in vivo osteogenic competence. Together, our findings establish periosteal organoids as a physiologically relevant and functionally competent regenerative platform, providing a versatile system for modeling skeletal repair and stem cell\u2010niche interactions.<\/jats:p>","DOI":"10.1002\/advs.78380","type":"journal-article","created":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:53:46Z","timestamp":1792727626000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long\u2010Term Expandable Periosteal Organoids Reconstruct a Functional Regenerative Niche That Support Hematopoietic Stem Cell Maintenance and Bone Repair"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2851-6848","authenticated-orcid":false,"given":"Jia","family":"Qing","sequence":"first","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1094-2205","authenticated-orcid":false,"given":"Lanxin","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lipeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1937-9212","authenticated-orcid":false,"given":"Xin","family":"Gao","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences Chongqing Municipal Key Laboratory for Oral Biomedical Engineering of Higher Education Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4427-4017","authenticated-orcid":false,"given":"Zechuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenpeng","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixin","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4332-0878","authenticated-orcid":false,"given":"Yongsheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8651-2100","authenticated-orcid":false,"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"},{"name":"Beijing Key Laboratory for Intelligent Biomanufacturing and Regeneration of Craniofacial Tissues &amp; NHC Key Laboratory of Digital Stomatology  Beijing China"},{"name":"Institute of Advanced Clinical Medicine Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"issue":"2","key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1089\/ten.teb.2022.0115","article-title":"A Systematic Review of Bone Marrow Stromal Cells and Periosteum\u2010Derived Cells for Bone Regeneration","volume":"29","author":"Zhu J.","year":"2023","journal-title":"Tissue Engineering Part B: Reviews"},{"issue":"5","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.bone.2004.07.014","article-title":"Periosteum: Biology, Regulation, and Response to Osteoporosis Therapies","volume":"35","author":"Allen M. R.","year":"2004","journal-title":"Bone"},{"issue":"4","key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s00256-009-0849-9","article-title":"The Periosteum: What is it, where is it, and What Mimics it in its Absence?","volume":"39","author":"Dwek J. R.","year":"2010","journal-title":"Skeletal Radiology"},{"issue":"12","key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1002\/jor.22181","article-title":"Current Insights on the Regenerative Potential of the Periosteum: Molecular, Cellular, and Endogenous Engineering Approaches","volume":"30","author":"Colnot C.","year":"2012","journal-title":"Journal of Orthopaedic Research"},{"issue":"5","key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1007\/s11914-022-00737-8","article-title":"Periosteal Skeletal Stem and Progenitor Cells in Bone Regeneration","volume":"20","author":"Perrin S.","year":"2022","journal-title":"Current Osteoporosis Reports"},{"issue":"1","key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/s41467-018-03124-z","article-title":"Periosteum Contains Skeletal Stem Cells With High Bone Regenerative Potential Controlled by Periostin","volume":"9","author":"Duchamp de Lageneste O.","year":"2018","journal-title":"Nature Communications"},{"issue":"4","key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1038\/ncb3139","article-title":"The Suture Provides a Niche for Mesenchymal Stem Cells of Craniofacial Bones","volume":"17","author":"Zhao H.","year":"2015","journal-title":"Nature Cell Biology"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10526","article-title":"Stem Cells of the Suture Mesenchyme in Craniofacial Bone Development, Repair and Regeneration","volume":"7","author":"Maruyama T.","year":"2016","journal-title":"Nature Communications"},{"issue":"7980","key":"e_1_2_11_10_1","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/s41586-023-06526-2","article-title":"A Multi\u2010Stem Cell Basis for Craniosynostosis and Calvarial Mineralization","volume":"621","author":"Bok S.","year":"2023","journal-title":"Nature"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.58534","article-title":"Heterogeneity of Murine Periosteum Progenitors Involved in Fracture Healing","volume":"10","author":"Matthews B. G.","year":"2021","journal-title":"Elife"},{"issue":"7725","key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/s41586-018-0554-8","article-title":"Discovery of a Periosteal Stem Cell Mediating Intramembranous Bone Formation","volume":"562","author":"Debnath S.","year":"2018","journal-title":"Nature"},{"issue":"6","key":"e_1_2_11_13_1","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.stem.2019.11.003","article-title":"Identification of Functionally Distinct Mx1+\u03b1SMA+ Periosteal Skeletal Stem Cells","volume":"25","author":"Ortinau L. C.","year":"2019","journal-title":"Cell Stem Cell"},{"issue":"11","key":"e_1_2_11_14_1","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1016\/j.stem.2022.10.002","article-title":"Bone Marrow and Periosteal Skeletal Stem\/Progenitor Cells Make Distinct Contributions to Bone Maintenance and Repair","volume":"29","author":"Jeffery E. C.","year":"2022","journal-title":"Cell Stem Cell"},{"issue":"30","key":"e_1_2_11_15_1","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202410243","article-title":"EGR1 Promotes Craniofacial Bone Regeneration via Activation of ALPL+PDGFD+ Periosteal Stem Cells","volume":"12","author":"Li Y.","year":"2025","journal-title":"Advanced Science"},{"issue":"9","key":"e_1_2_11_16_1","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/j.devcel.2024.03.019","article-title":"Fibrous Periosteum Repairs Bone Fracture and Maintains the Healed Bone Throughout Mouse Adulthood","volume":"59","author":"Liu Y. L.","year":"2024","journal-title":"Developmental Cell"},{"issue":"8033","key":"e_1_2_11_17_1","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1038\/s41586-024-07822-1","article-title":"The Periosteum Provides a Stromal Defence Against Cancer Invasion Into the Bone","volume":"634","author":"Nakamura K.","year":"2024","journal-title":"Nature"},{"issue":"7","key":"e_1_2_11_18_1","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1016\/j.cell.2016.05.082","article-title":"Modeling Development and Disease with Organoids","volume":"165","author":"Clevers H.","year":"2016","journal-title":"Cell"},{"issue":"11","key":"e_1_2_11_19_1","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/s41576-018-0051-9","article-title":"Progress and Potential in Organoid Research","volume":"19","author":"Rossi G.","year":"2018","journal-title":"Nature Reviews Genetics"},{"issue":"10","key":"e_1_2_11_20_1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/s41580-020-0259-3","article-title":"Human Organoids: Model Systems for Human Biology and Medicine","volume":"21","author":"Kim J.","year":"2020","journal-title":"Nature Reviews Molecular Cell Biology"},{"issue":"8","key":"e_1_2_11_21_1","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1038\/s41556-019-0360-z","article-title":"Patient\u2010Derived Organoids From Endometrial Disease Capture Clinical Heterogeneity and are Amenable to Drug Screening","volume":"21","author":"Boretto M.","year":"2019","journal-title":"Nature Cell Biology"},{"key":"e_1_2_11_22_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2021.120820","article-title":"Patterned, Organoid\u2010Based Cartilaginous Implants Exhibit Zone Specific Functionality Forming Osteochondral\u2010Like Tissues","volume":"273","author":"Hall G. N.","year":"2021","journal-title":"Biomaterials"},{"issue":"2","key":"e_1_2_11_23_1","article-title":"Developmentally Engineered Callus Organoid Bioassemblies Exhibit Predictive In Vivo Long Bone Healing","volume":"7","author":"Hall G. N.","year":"2020","journal-title":"Advancement of Science"},{"key":"e_1_2_11_24_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.actbio.2022.10.037","article-title":"Engineering Bone\u2010Forming Biohybrid Sheets Through the Integration of Melt Electrowritten Membranes and Cartilaginous Microspheroids","volume":"165","author":"Hall G. N.","year":"2023","journal-title":"Acta Biomaterialia"},{"issue":"4","key":"e_1_2_11_25_1","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ad6e1a","article-title":"Laser\u2010Assisted Bioprinting of Targeted Cartilaginous Spheroids for High Density Bottom\u2010Up Tissue Engineering","volume":"16","author":"Hall G. N.","year":"2024","journal-title":"Biofabrication"},{"issue":"2","key":"e_1_2_11_26_1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1039\/D4BM01181C","article-title":"Fast bone regeneration using injectable fully biomimetic organoids with biomineralized and organic microenvironments","volume":"13","author":"Zheng R.","year":"2025","journal-title":"Biomaterials Science"},{"issue":"3","key":"e_1_2_11_27_1","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.cell.2023.12.012","article-title":"Human Fetal Brain Self\u2010Organizes Into Long\u2010Term Expanding Organoids","volume":"187","author":"Hendriks D.","year":"2024","journal-title":"Cell"},{"issue":"6194","key":"e_1_2_11_28_1","doi-asserted-by":"crossref","DOI":"10.1126\/science.1247125","article-title":"Organogenesis in a Dish: Modeling Development and Disease Using Organoid Technologies","volume":"345","author":"Lancaster M. A.","year":"2014","journal-title":"Science"},{"issue":"7308","key":"e_1_2_11_29_1","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1038\/nature09262","article-title":"Mesenchymal and Haematopoietic Stem Cells Form a Unique Bone Marrow Niche","volume":"466","author":"M\u00e9ndez\u2010Ferrer S.","year":"2010","journal-title":"Nature"},{"issue":"5","key":"e_1_2_11_30_1","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1016\/j.celrep.2013.03.041","article-title":"Self\u2010Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion","volume":"3","author":"Isern J.","year":"2013","journal-title":"Cell Reports"},{"issue":"7","key":"e_1_2_11_31_1","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1084\/jem.20122252","article-title":"PDGFR\u03b1 and CD51 Mark Human Nestin+ Sphere\u2010Forming Mesenchymal Stem Cells Capable of Hematopoietic Progenitor Cell Expansion","volume":"210","author":"Pinho S.","year":"2013","journal-title":"Journal of Experimental Medicine"},{"issue":"26","key":"e_1_2_11_32_1","doi-asserted-by":"crossref","first-page":"7394","DOI":"10.1016\/j.cell.2024.10.044","article-title":"Long\u2010Term In\u00a0Vitro Expansion of a Human Fetal Pancreas Stem Cell That Generates All Three Pancreatic Cell Lineages","volume":"187","author":"Andersson\u2010Rolf A.","year":"2024","journal-title":"Cell"},{"issue":"2","key":"e_1_2_11_33_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.stem.2023.12.008","article-title":"Human Conjunctiva Organoids to Study Ocular Surface Homeostasis and Disease","volume":"31","author":"Bannier\u2010H\u00e9laou\u00ebt M.","year":"2024","journal-title":"Cell Stem Cell"},{"issue":"6","key":"e_1_2_11_34_1","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/j.cell.2018.11.013","article-title":"Long\u2010Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids","volume":"175","author":"Hu H.","year":"2018","journal-title":"Cell"},{"issue":"5","key":"e_1_2_11_35_1","doi-asserted-by":"crossref","DOI":"10.1093\/stmcls\/sxaf020","article-title":"Pdgfrbeta Marks Distinct Mesenchymal and Pericyte Populations Within the Periosteum With Overlapping Cellular Features","volume":"43","author":"Wang Z.","year":"2025","journal-title":"Stem Cells"},{"issue":"7","key":"e_1_2_11_36_1","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1038\/s41593-024-01678-4","article-title":"Distinct Origin and Region\u2010Dependent Contribution of Stromal Fibroblasts to Fibrosis Following Traumatic Injury in Mice","volume":"27","author":"Holl D.","year":"2024","journal-title":"Nature Neuroscience"},{"issue":"1","key":"e_1_2_11_37_1","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1038\/s41467-020-17740-1","article-title":"Single\u2010Cell Analysis Uncovers Fibroblast Heterogeneity and Criteria for Fibroblast and Mural Cell Identification and Discrimination","volume":"11","author":"Muhl L.","year":"2020","journal-title":"Nature Communications"},{"issue":"7","key":"e_1_2_11_38_1","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1042\/BCJ20200675","article-title":"Computational and Experimental Characterization of the Novel ECM Glycoprotein SNED1 and Prediction of its Interactome","volume":"478","author":"Vallet S. D.","year":"2021","journal-title":"Biochemical Journal"},{"issue":"240","key":"e_1_2_11_39_1","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3007096","article-title":"Chitinase 3\u2010like 1 Suppresses Injury and Promotes Fibroproliferative Responses in Mammalian Lung Fibrosis","volume":"6","author":"Zhou Y.","year":"2014","journal-title":"Science Translational Medicine"},{"issue":"19","key":"e_1_2_11_40_1","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.182524","article-title":"Identification of Postn+ Periosteal Progenitor Cells With Bone Regenerative Potential","volume":"9","author":"Yin B.","year":"2024","journal-title":"JCI Insight"},{"issue":"15","key":"e_1_2_11_41_1","doi-asserted-by":"crossref","first-page":"3852","DOI":"10.1016\/j.cell.2021.06.024","article-title":"Fibroblasts: Origins, Definitions, and Functions in Health and Disease","volume":"184","author":"Plikus M. V.","year":"2021","journal-title":"Cell"},{"issue":"17","key":"e_1_2_11_42_1","doi-asserted-by":"crossref","first-page":"14861","DOI":"10.1074\/jbc.M110.179762","article-title":"Dermatopontin Interacts with Fibronectin, Promotes Fibronectin Fibril Formation, and Enhances Cell Adhesion","volume":"286","author":"Kato A.","year":"2011","journal-title":"Journal of Biological Chemistry"},{"issue":"1","key":"e_1_2_11_43_1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1186\/s12967-023-04281-6","article-title":"Interactions Between MFAP5\u2009+\u2009Fibroblasts and Tumor\u2010Infiltrating Myeloid Cells Shape the Malignant Microenvironment of Colorectal Cancer","volume":"21","author":"Peng Z.","year":"2023","journal-title":"Journal of Translational Medicine"},{"issue":"1","key":"e_1_2_11_44_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1002\/jbmr.2598","article-title":"Fibrillin\u20101 Regulates Skeletal Stem Cell Differentiation by Modulating TGF\u03b2 Activity Within the Marrow Niche","volume":"31","author":"Smaldone S.","year":"2016","journal-title":"Journal of Bone and Mineral Research"},{"issue":"11","key":"e_1_2_11_45_1","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1038\/s12276-022-00899-6","article-title":"Neurovascular Coupling in Bone Regeneration","volume":"54","author":"Qin Q.","year":"2022","journal-title":"Experimental & Molecular Medicine"},{"issue":"6","key":"e_1_2_11_46_1","first-page":"30","article-title":"Revascularization and Angiogenesis for Bone Bioengineering in the Craniofacial Region: A Review","volume":"34","author":"Al\u2010Fotawi R.","year":"2023","journal-title":"Journal of Materials Science: Materials in Medicine"},{"issue":"24","key":"e_1_2_11_47_1","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202501254","article-title":"Dynamic GelMA\/DNA Dual\u2010Network Hydrogels Promote Woven Bone Organoid Formation and Enhance Bone Regeneration","volume":"37","author":"Zhu M. R.","year":"2025","journal-title":"Advanced Materials"},{"issue":"30","key":"e_1_2_11_48_1","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202470239","article-title":"Engineering Large\u2010Scale Self\u2010Mineralizing Bone Organoids with Bone Matrix\u2010Inspired Hydroxyapatite Hybrid Bioinks (Adv. Mater. 30\/2024)","volume":"36","author":"Wang J.","year":"2024","journal-title":"Advanced Materials"},{"key":"e_1_2_11_49_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2022.121741","article-title":"High\u2010Efficient Engineering of Osteo\u2010Callus Organoids for Rapid Bone Regeneration Within One Month","volume":"288","author":"Xie C.","year":"2022","journal-title":"Biomaterials"},{"issue":"2","key":"e_1_2_11_50_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/03913988231220844","article-title":"Osteogenic Organoid for Bone Regeneration: Healing of Bone Defect in Congenital Pseudoarthrosis of the Tibia","volume":"47","author":"Cardier J. E.","year":"2024","journal-title":"The International Journal of Artificial Organs"},{"issue":"1","key":"e_1_2_11_51_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mbm.2023.100009","article-title":"Stiffness Matters: A Soft Bone Marrow Organoid Rejuvenates Hematopoietic Stem Cells","volume":"1","author":"Zhang X. Y.","year":"2023","journal-title":"Mechanobiology in Medicine"},{"issue":"8","key":"e_1_2_11_52_1","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1002\/jbmr.4616","article-title":"Skeletal Stem\/Progenitor Cells in Periosteum and Skeletal Muscle Share a Common Molecular Response to Bone Injury","volume":"37","author":"Julien A.","year":"2022","journal-title":"Journal of Bone and Mineral Research"},{"issue":"9","key":"e_1_2_11_53_1","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.molmed.2017.07.003","article-title":"HSC Niche Biology and HSC Expansion Ex Vivo","volume":"23","author":"Kumar S.","year":"2017","journal-title":"Trends in Molecular Medicine"},{"issue":"7763","key":"e_1_2_11_54_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/s41586-019-1244-x","article-title":"Long\u2010Term Ex Vivo Haematopoietic\u2010Stem\u2010Cell Expansion Allows Nonconditioned Transplantation","volume":"571","author":"Wilkinson A. C.","year":"2019","journal-title":"Nature"},{"issue":"12","key":"e_1_2_11_55_1","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1016\/j.stem.2025.10.009","article-title":"Macro\u2010Scale, Scaffold\u2010Assisted Model of the Human Bone Marrow Endosteal Niche Using hiPSC\u2010Vascularized Osteoblastic Organoids","volume":"32","author":"Li Q.","year":"2025","journal-title":"Cell Stem Cell"},{"issue":"5","key":"e_1_2_11_56_1","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/s41592-024-02172-2","article-title":"Generation of Complex Bone Marrow Organoids From Human Induced Pluripotent Stem Cells","volume":"21","author":"Frenz\u2010Wiessner S.","year":"2024","journal-title":"Nature Methods"},{"issue":"2","key":"e_1_2_11_57_1","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1158\/2159-8290.CD-22-0199","article-title":"Human Bone Marrow Organoids for Disease Modeling, Discovery, and Validation of Therapeutic Targets in Hematologic Malignancies","volume":"13","author":"Khan A. O.","year":"2023","journal-title":"Cancer Discovery"},{"issue":"4","key":"e_1_2_11_58_1","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.stem.2023.03.005","article-title":"Harnessing Matrix Stiffness to Engineer a Bone Marrow Niche for Hematopoietic Stem Cell Rejuvenation","volume":"30","author":"Zhang X.","year":"2023","journal-title":"Cell Stem Cell"},{"issue":"2","key":"e_1_2_11_59_1","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.cell.2007.08.025","article-title":"Self\u2010Renewing Osteoprogenitors in Bone Marrow Sinusoids Can Organize a Hematopoietic Microenvironment","volume":"131","author":"Sacchetti B.","year":"2007","journal-title":"Cell"},{"issue":"3","key":"e_1_2_11_60_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.stem.2015.02.019","article-title":"Mesenchymal Cell Contributions to the Stem Cell Niche","volume":"16","author":"Kfoury Y.","year":"2015","journal-title":"Cell Stem Cell"},{"key":"e_1_2_11_61_1","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/s41586-019-1104-8","article-title":"The Bone Marrow Microenvironment at Single\u2010Cell Resolution","volume":"569","author":"Tikhonova A. N.","year":"2019","journal-title":"Nature"},{"issue":"1","key":"e_1_2_11_62_1","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/s41556-019-0439-6","article-title":"Combined Single\u2010Cell and Spatial Transcriptomics Reveal the Molecular, Cellular and Spatial Bone Marrow Niche Organization","volume":"22","author":"Baccin C.","year":"2020","journal-title":"Nature Cell Biology"},{"issue":"5","key":"e_1_2_11_63_1","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.70078","article-title":"fastp 1.0: An Ultra\u2010Fast All\u2010Round Tool for FASTQ Data Quality Control and Preprocessing","volume":"4","author":"Chen S. F.","year":"2025","journal-title":"iMeta"},{"key":"e_1_2_11_64_1","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14049","article-title":"Massively Parallel Digital Transcriptional Profiling of Single Cells","volume":"8","author":"Zheng G. X. Y.","year":"2017","journal-title":"Nature Communications"},{"issue":"2","key":"e_1_2_11_65_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/s41587-023-01767-y","article-title":"Dictionary Learning for Integrative, Multimodal and Scalable Single\u2010Cell Analysis","volume":"42","author":"Hao Y.","year":"2024","journal-title":"Nature Biotechnology"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78380","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.78380","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.78380","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:53:55Z","timestamp":1792727635000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.78380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,22]]},"references-count":64,"alternative-id":["10.1002\/advs.78380"],"URL":"https:\/\/doi.org\/10.1002\/advs.78380","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,22]]},"assertion":[{"value":"2026-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e78380"}}