{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T08:45:53Z","timestamp":1776761153166,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD2402900"],"award-info":[{"award-number":["2024YFD2402900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Insulin\u2010like androgenic gland (IAG) hormone is a central regulator of sex differentiation in decapod crustaceans. However, the tissue\u2010specific molecular interaction networks governing its gonadal functions remain poorly characterized. Through protein pull\u2010down assays, 279 candidate IAG\u2010interacting proteins were captured from gonadal tissues (androgenic gland [AG], testis and ovary) of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cherax quadricarinatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    followed by GO and KEGG enrichment analyses. Subsequently, 32 high\u2010confidence interactors were identified via dimeric complex structures prediction using a composite reliability threshold (ipTM + pTM &gt;\u20090.75). For eight key candidates, interacting amino acid residues and binding energies were further predicted with AlphaFold3. The AG harbored the largest number of interactors, showing significant enrichment in metabolic pathways. Notably, interactions with ubiquitin\u2010like modifier\u2010activating enzyme 1 (UBA1), heat shock protein (HSP) and histone\u2010lysine N\u2010methyltransferase (HKMT) in male gonadal tissues (AG and testis) suggest potential epigenetic regulatory roles for IAG. Conversely, in the ovary, IAG interacted with endoribonuclease Dicer (DICER1) and casein kinase II (CK2), implying putative post\u2010transcriptional and post\u2010translational mechanisms for inhibiting female maturation. This study reveals the tissue\u2010specific complexity of the IAG interactome in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cherax quadricarinatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    gonads, elucidating its potential dual roles in regulating metabolic processes in the AG and modulating post\u2010transcriptional\/post\u2010translational events in the ovary. These findings provide novel insights into the molecular mechanisms of crustacean sex development and identify new targets for sex\u2010control strategies in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cherax quadricarinatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/age.70095","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T12:04:30Z","timestamp":1774353870000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tissue\u2010Specific\n                    <scp>IAG<\/scp>\n                    Interactome in\n                    <scp>\n                      <i>Cherax quadricarinatus<\/i>\n                    <\/scp>\n                    Gonads"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2002-9754","authenticated-orcid":false,"given":"Honglin","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Institute of Hydrobiology Zhejiang Academy of Agricultural Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Institute of Hydrobiology Zhejiang Academy of Agricultural Sciences  Hangzhou China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University Ministry of Agriculture  Shanghai 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