{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:49:01Z","timestamp":1782208141131,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n A prior genome\u2010wide association (GWA;\n N<\/jats:italic>\n \u2009=\u2009200) including Thoroughbreds and stock horses implicated chromosome 6 (NC_009149.3) in owner\u2010reported equine anhidrosis. A missense variant in\n KCNE4<\/jats:italic>\n (NC_009149.3:g.11813731A>G) was proposed as a risk allele, although its association with anhidrosis was not reported. Variant annotation and protein modelling in the original study suggested the G allele conferred risk. We reported no association of the G allele with anhidrosis in 50 horses phenotyped by an intradermal terbutaline sweat test (ITST); all horses produced sweat regardless of genotype. It later appeared the A allele was instead suggested to confer disease risk. To reassess this, we genotyped 20 ITST\u2010tested Thoroughbreds including 9 with partial or complete anhidrosis. The\n KCNE4<\/jats:italic>\n A allele was not associated with phenotype when analyzed as a binary trait (\n p<\/jats:italic>\n \u2009=\u20090.16) or when classifying affected horses as having either partial or complete anhidrosis (\n p<\/jats:italic>\n \u2009=\u20090.21). The locus was also uninformative in four clinical cases (AA\u2009=\u20091, AG\u2009=\u20091, GG\u2009=\u20092). Reasoning that a true risk allele should be in linkage disequilibrium (LD) with the associated GWA SNV (AX\u2010103822151; rs68656009), we evaluated whole\u2010genome sequence (\n N<\/jats:italic>\n \u2009=\u20092) from the initial publication. Both case and control were homozygous AA at the putative risk locus; the case was heterozygous at the GWA SNV. In public data (\n N<\/jats:italic>\n \u2009=\u2009897), LD between loci was low (r\n 2<\/jats:sup>\n \u2009=\u20090.19). In 369 Thoroughbreds, LD was 0.58. Restricting to Thoroughbreds in the original GWA (\n N<\/jats:italic>\n \u2009=\u200985), we found no association of chromosome 6 with anhidrosis. Collectively, these data do not support a role of the\n KCNE4<\/jats:italic>\n variant or the GWA SNV in equine anhidrosis.\n <\/jats:p>","DOI":"10.1002\/age.70109","type":"journal-article","created":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T22:28:41Z","timestamp":1777242521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Additional Evidence Fails to Associate Variation in\n \n KCNE4<\/i>\n <\/scp>\n With Equine Anhidrosis"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5438-8555","authenticated-orcid":false,"given":"Jessica L.","family":"Petersen","sequence":"first","affiliation":[{"name":"Department of Animal Science University of Nebraska\u2010Lincoln Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5924-0234","authenticated-orcid":false,"given":"Carrie J.","family":"Finno","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction University of California, Davis School of Veterinary Medicine Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-73645-9"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003440"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1497817"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118939956.ch73"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-09111-7"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.758366"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.236.10.1091"},{"key":"e_1_2_5_9_1","volume-title":"Genetic Mutation Found in Anhidrotic Horses","author":"Kentucky Equine Research","year":"2022"},{"key":"e_1_2_5_10_1","unstructured":"Li H.2013.Aligning Sequence Reads Clone Sequences and Assembly Contigs With BWA\u2010MEM arXiv:1303.3997v2 [q\u2010bio.GN]."},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516408X322409"},{"key":"e_1_2_5_12_1","volume-title":"The Genetics of Anhidrosis","author":"Mallicote M.","year":"2023"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1987.tb02001.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002451"},{"key":"e_1_2_5_15_1","volume-title":"Blogs","author":"Moore 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Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70109","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/age.70109","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:13:46Z","timestamp":1782206026000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/age.70109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,26]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/age.70109"],"URL":"https:\/\/doi.org\/10.1002\/age.70109","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,26]]},"assertion":[{"value":"2025-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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