{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:48:56Z","timestamp":1782208136085,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20260102191JC"],"award-info":[{"award-number":["20260102191JC"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Circular RNAs (circRNAs) are increasingly recognized as key post\u2010transcriptional regulators. However, their functions within the avian hypothalamic\u2013pituitary\u2013ovarian (HPO) axis remain poorly understood. To investigate potential associations of circRNAs with breed differentiation and reproductive traits, we constructed cross\u2010tissue circRNA profiles through whole\u2010transcriptome sequencing of the HPO axis in Zi and Xianghai flying geese. A total of 90, 80, and 90 DE\u2010circRNAs were identified in the hypothalamus, ovary, and pituitary, respectively, corresponding to 248 non\u2010redundant DE\u2010circRNA IDs across the three tissues. Functional enrichment analysis revealed that their host genes were significantly enriched in reproduction\u2010related signaling pathways, including cAMP, PI3K\u2013Akt, FoxO, JAK\u2013STAT, GnRH, and VEGF signaling pathways. GO terms related to reproduction, reproductive process, and growth were significantly enriched, with protein interaction analysis identifying CYP19A1, FSHR, BMP15, and ESR1 as central network hubs. Furthermore, through systematic integration of cross\u2010tissue circRNA, miRNA, and mRNA expression profiles and a series of analytical steps including correlation\u2010based filtering and hypergeometric testing, we thereby constructed a cross\u2010tissue ceRNA network. Several functional modules were identified, including novel_circ_000988\u2013miR\u20101451\u2010y\/miR\u2010216\u2010x\/miR\u2010735\u2010x\u2013\n                    <jats:italic>IL20RA<\/jats:italic>\n                    , novel_circ_019479\u2013miR\u20101338\u2010y\/miR\u20101388\u2010y\u2013\n                    <jats:italic>GLRA2<\/jats:italic>\n                    , and novel_circ_010669\u2013miR\u2010143\u2010z\u2013\n                    <jats:italic>Med27<\/jats:italic>\n                    . Our results indicate that circRNAs may be associated with a cross\u2010tissue neuroendocrine\u2013ovarian regulatory network through putative ceRNA\u2010associated interactions, providing candidate molecular insights into reproductive trait variation in geese.\n                  <\/jats:p>","DOI":"10.1002\/age.70133","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T02:55:42Z","timestamp":1781578542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Circular\n                    <scp>RNAs<\/scp>\n                    Analysis Across the Hypothalamic\u2013Pituitary\u2013Ovarian Axis Identifies Regulatory Modules Shaping Reproductive Traits in Geese"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2106-2937","authenticated-orcid":false,"given":"Linhong","family":"Xie","sequence":"first","affiliation":[{"name":"College of Animal 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