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To this end, a SNP\u2010PCR sequencing\u2010based panel comprising 93 markers in one reaction was designed and investigated for use in Thoroughbred horses. Marker amplification performance and discriminating power were calculated against a population survey of 264 Thoroughbred whole blood samples from routine doping testing. The sensitivity of the SNP panel was tested on a dilution series and on a variety of matrices, including different types of blood specimen, hair and faeces. No genotyping errors due to allelic dropout were detected down to a starting concentration of 0.25\u2009ng\/\u03bcL in the dilution series, with both EDTA and lithium heparin anticoagulants performing comparably. Forensic \u2018crime scene\u2019 samples, such as blood on a blood tube label, also performed strongly, although others were more variable due to low DNA concentrations. A mock case of 10 \u2018suspects\u2019 and one test blood sample was performed blind by the analyst who correctly identified the source of the sample.<\/jats:p>","DOI":"10.1002\/age.70170","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:07:55Z","timestamp":1785334075000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Approaches to Thoroughbred Horse Identification in Diverse Sample Types Using Multiplexed\n                    <scp>SNP<\/scp>\n                    \u2010\n                    <scp>PCR<\/scp>\n                    Sequencing Panels"],"prefix":"10.1002","volume":"57","author":[{"given":"Jillian","family":"Maniego","sequence":"first","affiliation":[{"name":"LGC Ltd  Fordham Cambridgeshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0961-8849","authenticated-orcid":false,"given":"Caitlin","family":"Harding","sequence":"additional","affiliation":[{"name":"LGC Ltd  Fordham Cambridgeshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0810-9284","authenticated-orcid":false,"given":"Pamela","family":"Hincks","sequence":"additional","affiliation":[{"name":"LGC Ltd  Fordham Cambridgeshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6963-797X","authenticated-orcid":false,"given":"Jocelyn","family":"Habershon\u2010Butcher","sequence":"additional","affiliation":[{"name":"British Horseracing Authority  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1799-9899","authenticated-orcid":false,"given":"Edward","family":"Ryder","sequence":"additional","affiliation":[{"name":"LGC Ltd  Fordham Cambridgeshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.13408"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1365\u20102052.1997.00123.X"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1365\u2010294X.2004.02352.X"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.12357"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAB960"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/00087114.1979.10796788"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.FSIGSS.2009.08.161"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/HUMREP\/10.6.1609"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3996\/112014\u2010JFWM\u2010080"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13583"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0008645"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1292\/JVMS.09\u20100486"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa942"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2353\/JMOLDX.2007.060059"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010018\u20100199\u2010z"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PCBI.1008468"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12090890"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/RMB2.12675"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","unstructured":"Knaus B. 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