{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:24:32Z","timestamp":1787203472935,"version":"build-2736575974"},"reference-count":76,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018806","name":"Department of Science and Technology of Hubei Province","doi-asserted-by":"publisher","award":["HBZY2023B008"],"award-info":[{"award-number":["HBZY2023B008"]}],"id":[{"id":"10.13039\/501100018806","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sheep seasonal reproduction is regulated by photoperiod via melatonin. Indoleamine 2,3\u2010dioxygenase 2 (IDO2), an enzyme involved in tryptophan metabolism through the kynurenine pathway and belonging to the IDO family, may contribute to melatonin homeostasis, but its role in reproductive seasonality remains unclear. In this study, genome\u2010wide selection analyses combining\n                    <jats:italic>Z<\/jats:italic>\n                    (\n                    <jats:italic>F<\/jats:italic>\n                    <jats:sub>ST<\/jats:sub>\n                    ) and XP\u2010EHH between seasonal and non\u2010seasonal sheep identified a selection signature encompassing the\n                    <jats:italic>IDO2<\/jats:italic>\n                    region and revealed g.\u2212809T&gt;C as a functional candidate variant within this region. Experiments employing electrophoretic mobility shift assay (EMSA) and luciferase assays revealed that this site lies within the CLOCK transcription factor binding domain. The \u2212809C variant reduced promoter activity compared to \u2212809T (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Sheep with the CC genotype had higher plasma melatonin levels than TC\/TT counterparts (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). These findings suggest that the g.\u2212809C allele decreases\n                    <jats:italic>IDO2<\/jats:italic>\n                    transcription by reducing CLOCK binding affinity, thereby potentially affecting\n                    <jats:italic>IDO2<\/jats:italic>\n                    \u2010associated tryptophan\/melatonin metabolism and increasing melatonin levels, which may contribute to photoperiodic responses in sheep. This\n                    <jats:italic>IDO2<\/jats:italic>\n                    variation provides new insights into the genetic regulation of melatonin\u2010mediated reproductive seasonality and represents a potential marker for genetic improvement of reproductive seasonal traits in sheep.\n                  <\/jats:p>","DOI":"10.1002\/age.70173","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T01:46:44Z","timestamp":1785721604000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Functional\n                    <scp>SNP<\/scp>\n                    in the Promoter Region of Ovine\n                    <i>IDO2<\/i>\n                    Associated With Melatonin Levels via Affecting Transcription Activity by\n                    <scp>CLOCK<\/scp>"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7822-1784","authenticated-orcid":false,"given":"Xunping","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaxuan","family":"Chi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengya","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoying","family":"Tie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiguo","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry 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