{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:24:21Z","timestamp":1787203461717,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2019\/35\/B\/NZ5\/00775"],"award-info":[{"award-number":["2019\/35\/B\/NZ5\/00775"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CP23\/00072"],"award-info":[{"award-number":["CP23\/00072"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004895","name":"European Social Fund Plus","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Canine mammary tumours (CMTs) are one of the most frequently observed malignancies in female dogs. Many studies have investigated epigenetic changes in CMT at the nuclear level, yet the role of mtDNA methylation, particularly in the D\u2010loop region, remains largely unexplored. Given the significant importance of the D\u2010loop region in mtDNA replication and transcription, and the previous identification of CpG enrichment there, this preliminary study aimed to assess apparent methylation signals in the mitochondrial D\u2010loop region. We examined CpG, CHG and CHH methylation contexts in matched tumour and normal tissues from six German Shepherd dogs with histologically classified mammary carcinomas using enzymatic methyl\u2010sequencing and high\u2010throughput amplicon sequencing. Overall, CpG and CHH contexts exhibited greater variability between tumour and normal tissues than CHG, which remained relatively stable. Correlation analyses revealed a putative positive relationship between differences in CpG and CHH methylation (Spearman's\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.829,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.042). This might suggest a coordinated epigenetic modulation of these two methylation patterns in the carcinogenesis process, yet due to the limited study group (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) the hypothesis should be verified on a larger cohort. These findings demonstrate a plausible existence of intra\u2010individual epigenetic divergence at the mitochondrial level. To our knowledge, this is the first study to assess the influence of mtDNA methylation on CMT, and it supports further investigation of mitochondrial epigenetics in canine oncology.\n                  <\/jats:p>","DOI":"10.1002\/age.70178","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:14:51Z","timestamp":1785802491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Analysis of\n                    <scp>CpG<\/scp>\n                    ,\n                    <scp>CHG<\/scp>\n                    and\n                    <scp>CHH<\/scp>\n                    Methylation in the Mitochondrial Control Region of Dogs With Malignant Mammary Gland Tumours"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4716-5040","authenticated-orcid":false,"given":"Krzysztof","family":"Kowal","sequence":"first","affiliation":[{"name":"Institute of Biological Bases of Animal Production University of Life Sciences in Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaja","family":"Zi\u00f3\u0142kowska\u2010Twarowska","sequence":"additional","affiliation":[{"name":"Institute of Biological Bases of Animal Production University of Life Sciences in Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Tkaczyk\u2010Wliz\u0142o","sequence":"additional","affiliation":[{"name":"Institute of Biological Bases of Animal Production University of Life Sciences in Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8658-5502","authenticated-orcid":false,"given":"Carlos","family":"Vaamonde\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Reumatolog\u00eda (GIR), Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC), Sergas, Universidade de A Coru\u00f1a (UDC)  A Coru\u00f1a Spain"},{"name":"Grupo de Investigaci\u00f3n en Reumatolog\u00eda y Salud (GIR\u2010S) Centro Interdisciplinar de Qu\u00edmica y Biolog\u00eda (CICA), Universidade de A Coru\u00f1a (UDC)  A Coru\u00f1a Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Fern\u00e1ndez\u2010Moreno","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Reumatolog\u00eda (GIR), Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC), Sergas, Universidade de A Coru\u00f1a (UDC)  A Coru\u00f1a Spain"},{"name":"Grupo de Investigaci\u00f3n en Reumatolog\u00eda y Salud (GIR\u2010S) Centro Interdisciplinar de Qu\u00edmica y Biolog\u00eda (CICA), Universidade de A Coru\u00f1a (UDC)  A Coru\u00f1a Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3933-2400","authenticated-orcid":false,"given":"Brygida","family":"\u015alaska","sequence":"additional","affiliation":[{"name":"Institute of Biological Bases of Animal Production University of Life Sciences in Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","unstructured":"Amfim A. 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