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Gene editing of the myostatin (MSTN) gene represents a promising strategy to enhance muscle growth in cattle. In this study, MSTN\u2010mutated founder (F0) cows were used to generate F1 offspring via ovum pick\u2010up, in\u00a0vitro fertilization, and embryo transfer. Four F1 calves were born, all confirmed to be heterozygous for the MSTN mutation. Long\u2010term monitoring showed normal growth and no visible health abnormalities. Whole\u2010genome sequencing identified SNPs, INDELs, and structural variants, most with minimal predicted functional effects. Proteomic profiling of Longissimus dorsi muscle quantified 2947 proteins, revealing only subtle expression differences between MSTN\u2010mutated and wild\u2010type cattle. These results demonstrate stable inheritance and confirm that MSTN editing does not disrupt genome integrity or protein expression. Overall, our findings support the safety and utility of MSTN gene editing to improve livestock productivity for future food security.<\/jats:p>","DOI":"10.1002\/age.70192","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:48:09Z","timestamp":1788158889000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Safe and Stable Germline Transmission of\n                    <scp>MSTN<\/scp>\n                    Mutations in Cattle"],"prefix":"10.1002","volume":"57","author":[{"given":"Gyeong\u2010Min","family":"Gim","sequence":"first","affiliation":[{"name":"LARTBio Inc.  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