{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:42:00Z","timestamp":1792662120732,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Myostatin (MSTN), encoded by the\n                    <jats:italic>MSTN<\/jats:italic>\n                    gene, is a critical negative regulator of skeletal muscle mass. This study aims to identify and characterize the miRNAs involved in the development of the double\u2010muscling phenotype in\n                    <jats:italic>MSTN<\/jats:italic>\n                    \u2010deficient rabbits. We performed high\u2010throughput sequencing to analyze the miRNA expression profiles in gluteus maximus tissue from wild type (\n                    <jats:italic>MSTN<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    ) and\n                    <jats:italic>MSTN<\/jats:italic>\n                    \u2010KO (\n                    <jats:italic>MSTN<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    and\n                    <jats:italic>MSTN<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    inclusive) rabbits. Differentially expressed miRNAs (DEmiRNAs) were identified, and their potential target genes were predicted. Functional enrichment analysis of these target mRNAs was conducted using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to elucidate the involved biological pathways and regulatory networks. A total of 25 DEmiRNAs (13 downregulated and 12 upregulated, |log\n                    <jats:sub>2<\/jats:sub>\n                    FC|\u2009\u2265\u20091.0, adjusted\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and 1178 differentially expressed mRNAs (408 upregulated and 770 downregulated, |log\n                    <jats:sub>2<\/jats:sub>\n                    FC|\u2009\u2265\u20092.0, adjusted\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) were identified in\n                    <jats:italic>MSTN<\/jats:italic>\n                    \u2010KO compared to\n                    <jats:italic>MSTN<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    rabbits. Bioinformatics analysis revealed that the target genes of these DEmiRNAs were significantly enriched in key pathways governing muscle growth and metabolism, including the PI3K\u2010Akt signaling pathway, MAPK signaling pathway, and pathways related to ECM\u2010receptor interaction and insulin signaling. Notably, many predicted target mRNAs are expressed by genes that encode key inhibitors of myogenesis (e.g.,\n                    <jats:italic>HDAC4<\/jats:italic>\n                    ) and major extracellular matrix components (e.g.,\n                    <jats:italic>COL4A3, POSTN<\/jats:italic>\n                    ). Our results demonstrate that\n                    <jats:italic>MSTN<\/jats:italic>\n                    deficiency induces a distinct and widespread change in the miRNA expression landscape of skeletal muscle.\n                  <\/jats:p>","DOI":"10.1002\/age.70193","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:21:24Z","timestamp":1788870084000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Analysis of\n                    <scp>miRNAs<\/scp>\n                    and Predicted Protein Interaction Networks in Skeletal Muscle Development of Myostatin\u2010Deficient Rabbits"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3520-8437","authenticated-orcid":false,"given":"Zhi\u2010Xian","family":"Song","sequence":"first","affiliation":[{"name":"Medical College Yangzhou University  Yangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1091-1913","authenticated-orcid":false,"given":"Paing Oo","family":"Kyaw","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu co\u2010Innovation Center of Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu Interdisciplinary Center for Zoonoses and Biosafety Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University  Yangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-6268","authenticated-orcid":false,"given":"Muhammad","family":"Farhab","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu co\u2010Innovation Center of Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu Interdisciplinary Center for Zoonoses and Biosafety Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University  Yangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9819-0073","authenticated-orcid":false,"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Animal Husbandry and Veterinary Medicine Jiangsu Vocational College of Agriculture and Forestry  Jurong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9674-0235","authenticated-orcid":false,"given":"Jingyan","family":"Liang","sequence":"additional","affiliation":[{"name":"Medical College Yangzhou University  Yangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5817-4247","authenticated-orcid":false,"given":"Yu\u2010Guo","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu co\u2010Innovation Center of Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu Interdisciplinary Center for Zoonoses and Biosafety Yangzhou University  Yangzhou People's Republic of China"},{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University  Yangzhou People's 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