{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:42:18Z","timestamp":1792662138080,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000733","name":"Universities Federation for Animal Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000733","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/13600\u20100"],"award-info":[{"award-number":["2018\/13600\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"Svenska Forskningsr\u00e5det Formas","doi-asserted-by":"publisher","award":["2021\u201000532"],"award-info":[{"award-number":["2021\u201000532"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Environmental stressors in commercial poultry systems can negatively affect bird welfare, although individuals vary considerably in their responses. Neuroplasticity within the hippocampus, measured through the density of doublecortin\u2010positive (DCX\n                    <jats:sup>+<\/jats:sup>\n                    ) neurons, provides a potential biomarker of stress experience in laying hens. However, the genetic basis underlying variation in this biomarker remains poorly understood. A total of 42 H&amp;N and Hy\u2010Line Brown hens housed in a multitier free range and enriched cage system, respectively, were genotyped using Genotyping by Sequencing, yielding over 200\u2009000 SNP markers after initial filtering. Hippocampal tissue sections were immunostained for DCX to quantify the density of highly plastic neurons. A genome\u2010wide association analysis identified 19 genomic regions across eight chromosomes within the top 1% of windows explaining the greatest proportion of genetic variance in the neuroplasticity phenotype. Within \u00b1100\u2009kb of these regions, 39 annotated genes were identified, several of which are involved in cellular regulation and genetic information processing pathways. Notably,\n                    <jats:italic>PIK3R6<\/jats:italic>\n                    ,\n                    <jats:italic>VPS37D<\/jats:italic>\n                    ,\n                    <jats:italic>STX1A<\/jats:italic>\n                    ,\n                    <jats:italic>BAZ1B<\/jats:italic>\n                    ,\n                    <jats:italic>HGH1<\/jats:italic>\n                    ,\n                    <jats:italic>MAF1<\/jats:italic>\n                    ,\n                    <jats:italic>MAPK15<\/jats:italic>\n                    , and\n                    <jats:italic>PIT54<\/jats:italic>\n                    emerged as positional candidate genes potentially contributing to variation in stress responsiveness. These findings provide preliminary insight into the genetic architecture of hippocampal neuroplasticity in laying hens and highlight candidate genes that may contribute to individual differences in stress response, with potential implications for breeding strategies aimed at improving poultry welfare.\n                  <\/jats:p>","DOI":"10.1002\/age.70202","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:15:46Z","timestamp":1789049746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome\u2010Wide Association Analysis of Hippocampal Neuroplasticity as an Indicator of Stress Responsiveness in\u00a0Laying Hens (\n                    <i>Gallus gallus domesticus<\/i>\n                    )"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1853-0264","authenticated-orcid":false,"given":"Lindsay","family":"Neuert","sequence":"first","affiliation":[{"name":"Department of Animal Biosciences, Centre for Genetic Improvement of Livestock University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vern","family":"Osborne","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, Centre for Genetic Improvement of Livestock University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena A.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"School of Psychology Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7214-9184","authenticated-orcid":false,"given":"F\u00e1bio","family":"P\u00e9rtille","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology, Physiology and Environmental Toxicology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1935-5875","authenticated-orcid":false,"given":"Carlos Guerrero","family":"Bosagna","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology, Physiology and Environmental Toxicology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Boswell","sequence":"additional","affiliation":[{"name":"School of Natural and Environmental Sciences Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom V.","family":"Smulders","sequence":"additional","affiliation":[{"name":"School of Psychology Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine F.","family":"Baes","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, Centre for Genetic Improvement of Livestock University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3485-0201","authenticated-orcid":false,"given":"Bayode O.","family":"Makanjuola","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, Centre for Genetic Improvement of Livestock University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201803127"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201059940\u20101"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201018504\u20101"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000362917"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23661"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2007.02.002"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm308"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12141803"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13742\u2010015\u20100047\u20108"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u20109\u2010516"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/neuro.22.002.2009"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.11.031"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102826.2011.02214.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201043584\u2010x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti519"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.250951"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2022.110601"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2023.105451"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.865743"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2015.11.014"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac958"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.1999.tb08103.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.c.30257"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26929"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102156\u201011\u2010103"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0953\u2010816x.2003.03123.x"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601\u20105223.2008.02043.x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz369"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000000838"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2009\u201000483"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12030405"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2018.05.002"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477654"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3598\u201015.2016"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.4064"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/35103535"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201910812"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105704"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.6.520"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.03.059"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23476"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1700398"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.11.011"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3333\u201016.2017"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70202","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/age.70202","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:20:16Z","timestamp":1792660816000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/age.70202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,10]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/age.70202"],"URL":"https:\/\/doi.org\/10.1002\/age.70202","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,10]]},"assertion":[{"value":"2026-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70202"}}