{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:42:14Z","timestamp":1792662134258,"version":"4.5.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF1000600"],"award-info":[{"award-number":["2021YFF1000600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    MicroRNAs (miRNAs) are key posttranscriptional regulators of gene expression and play important roles in physiological responses to hypoxia, particularly in the cardiovascular system. However, the regulatory roles of miRNAs in cardiopulmonary tissues during high\u2010altitude adaptation remain poorly understood. Tibetan pigs are a high\u2010altitude\u2010adapted breed with strong hypoxia tolerance, making them an ideal model for investigating the molecular mechanisms underlying high\u2010altitude adaptation. In this study, we compared miRNA expression profiles between Tibetan pigs and low\u2010altitude Meishan pigs and integrated multi\u2010omics data to investigate miRNA\u2010mediated regulatory networks, with a particular focus on target genes and 3'UTR genetic variation. Small RNA and mRNA sequencing of heart and lung tissues identified 70 differentially expressed miRNAs and 453 candidate target genes associated with hypoxia adaptation, which were significantly enriched in pathways such as vascular smooth muscle contraction. Integrative analysis of whole\u2010genome resequencing data from 20 Tibetan pigs and 17 Meishan pigs further revealed that genetic variants within 3'UTR regions may contribute to the modulation of miRNA\u2010mRNA interactions. Notably, we identified a highly differentiated SNP within the predicted miR\u2010221\u20105p binding site in the 3'UTR of\n                    <jats:italic>RAB17<\/jats:italic>\n                    , suggesting a potential role of this variant in posttranscriptional regulation. Dual\u2010luciferase reporter assays and functional validation further supported a regulatory interaction between miR\u2010221\u20105p and the\n                    <jats:italic>RAB17<\/jats:italic>\n                    3'UTR. Collectively, these findings provide an integrative multi\u2010omics framework for elucidating candidate miRNA regulatory networks associated with high\u2010altitude adaptation and highlight the potential contribution of miRNA\u2010mediated posttranscriptional regulation and 3'UTR genetic variation to high\u2010altitude adaptation in Tibetan pigs.\n                  <\/jats:p>","DOI":"10.1002\/age.70205","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:24:18Z","timestamp":1789730658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative Multi\u2010Omics Analysis Identifies Candidate\n                    <scp>MicroRNA<\/scp>\n                    Regulatory Networks Associated With High\u2010Altitude Adaptation in Tibetan Pigs"],"prefix":"10.1002","volume":"57","author":[{"given":"Xiaolu","family":"Qu","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"},{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianli","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"},{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"},{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanjie","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"},{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Bao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingwang","family":"Lai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9892-3887","authenticated-orcid":false,"given":"Yunlong","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8248-9126","authenticated-orcid":false,"given":"Guoqiang","family":"Yi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genome and Multi\u2010Omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China"},{"name":"Bama Yao Autonomous County Rural Revitalization Research Institute  Hechi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1187"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12020342"},{"key":"e_1_2_11_4_1","first-page":"291","article-title":"Exosomes 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