{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:57:57Z","timestamp":1790927877042,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["047\/2012"],"award-info":[{"award-number":["047\/2012"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017654","name":"Banco Nacional de Desenvolvimento Econ\u00f4mico e Social","doi-asserted-by":"publisher","award":["2.318.697.0001"],"award-info":[{"award-number":["2.318.697.0001"]}],"id":[{"id":"10.13039\/501100017654","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307170\/2021\u20107"],"award-info":[{"award-number":["307170\/2021\u20107"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307154\/2021\u20101"],"award-info":[{"award-number":["307154\/2021\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The order Passeriformes, the most speciose avian order, includes over 6000 species (~60% of all birds). The Amazon Basin alone hosts &gt;\u2009900 bird species, with Passeriformes representing &gt;\u2009500 of them. The family Thamnophilidae comprises ~200 insectivorous species, accounting for 15% of Neotropical passerine richness. Within Thamnophilinae,\n                    <jats:italic>Isleria<\/jats:italic>\n                    is the least speciose genus, containing only two species. It was erected after phylogenetic analyses revealed\n                    <jats:italic>Myrmotherula<\/jats:italic>\n                    as non\u2010monophyletic. To advance knowledge of chromosomal evolution in Passeriformes, we analyzed the karyotype of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Isleria hauxwelli<\/jats:italic>\n                    <\/jats:styled-content>\n                    using molecular cytogenetics. We found a diploid number of 2n\u2009=\u200984 and a fundamental number (FN) of 92. The BOE\u20107 probe, corresponding to the ancestral fusion of GGA\u20109 with two microchromosomes, maintained that configuration without undergoing fission to produce IHA\u20105. The BOE\u201014 probe, typically fragmented in all cytogenomically characterized Passeriformes, corresponded to two distinct GGA segments: the R3 portion and a region of unknown homology. This is the first documentation of such chromosomal organization in passerines. The absence of these fissions suggests a possible autapomorphic chromosomal condition within Thamnophilidae. Additionally, a potential fission involving microchromosomes homeologous to probes BOE\u201015 and BOE\u201016 is suggested: while these probes mark three microchromosome pairs in GGA, they mark four pairs in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>I. hauxwelli<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2014a feature not yet observed in other passerines. As most Thamnophilidae remain cytogenetically uncharacterized, systematic study of this family is essential to determine whether these rearrangements represent synapomorphies, autapomorphies, or convergent events.\n                  <\/jats:p>","DOI":"10.1002\/age.70210","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:50:12Z","timestamp":1790927412000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Chromosomal Organization of\n                    <i>Isleria hauxwelli<\/i>\n                    and Its Meaning for the Karyotypic Evolution in Thamnophilidae (Passeriformes)"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0066-8994","authenticated-orcid":false,"given":"Paulo Victor De Moraes","family":"Ferreira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00e9tica, Centro de Estudos Avan\u00e7ados da Biodiversidade, ICB Universidade Federal do Par\u00e1, PCT\u2010Guam\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita Fernanda Augusto","family":"Ribas","sequence":"additional","affiliation":[{"name":"Instituto de Biodiversidade e Florestas Universidade Federal do Oeste do Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"},{"name":"Laborat\u00f3rio de Gen\u00e9tica e Biodiversidade, Instituto de Ci\u00eancias da Educa\u00e7\u00e3o Universidade Federal do Oeste do Par\u00e1  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ane Nobre","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00e9tica, Centro de Estudos Avan\u00e7ados da Biodiversidade, ICB Universidade Federal do Par\u00e1, PCT\u2010Guam\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Guilherme","family":"de Silva Tembra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00e9tica, Centro de Estudos Avan\u00e7ados da Biodiversidade, ICB Universidade Federal do Par\u00e1, PCT\u2010Guam\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5399-8308","authenticated-orcid":false,"given":"Milla","family":"de Andrade Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00e9tica, Centro de Estudos Avan\u00e7ados da Biodiversidade, ICB Universidade Federal do Par\u00e1, PCT\u2010Guam\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia C. M.","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9372-1381","authenticated-orcid":false,"given":"Malcolm Andrew","family":"Ferguson\u2010Smith","sequence":"additional","affiliation":[{"name":"Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengtang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology  Zibo Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1516-2734","authenticated-orcid":false,"given":"Cleusa Yoshiko","family":"Nagamachi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00e9tica, Centro de Estudos Avan\u00e7ados da Biodiversidade, ICB Universidade Federal do Par\u00e1, PCT\u2010Guam\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2951-8877","authenticated-orcid":false,"given":"Julio Cesar","family":"Pieczarka","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00e9tica, Centro de Estudos Avan\u00e7ados da Biodiversidade, ICB Universidade Federal do Par\u00e1, PCT\u2010Guam\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen\u20102023\u20100096"},{"key":"e_1_2_10_3_1","volume-title":"Birds of the World","author":"Billerman S. M.","year":"2024"},{"key":"e_1_2_10_4_1","unstructured":"BirdLife International.2025.\u201cImportant Bird Areas Factsheet: Isla Diego de Almagro.\u201dhttps:\/\/datazone.birdlife.org\/site\/factsheet\/isla\u2010diego\u2010de\u2010almagro."},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.11646\/zootaxa.3195.1.5","article-title":"Isleria, a New Genus of Antwren (Aves: Passeriformes: Thamnophilidae)","volume":"3195","author":"Bravo G. A.","year":"2012","journal-title":"Zootaxa"},{"key":"e_1_2_10_6_1","unstructured":"CEMAVE.1994.\u201cManual de Anilhamento de Aves Silvestres.\u201dhttps:\/\/www.gov.br\/icmbio\/pt\u2010br\/centrais\u2010de\u2010conteudo\/publicacoes\/publicacoes\u2010diversas\/fauna\u2010e\u2010flora\/manual\u2010de\u2010anilhamento\u2010de\u2010aves\u2010silvestres\/manual_de_anilhamento_de_aves_silvestres.pdf."},{"key":"e_1_2_10_7_1","first-page":"31","article-title":"Chromosome Complements and Karyotype Evolution in Apodiformes, Gruiformes and Passeriformes (Aves)","volume":"2","author":"Lucca E. J.","year":"1977","journal-title":"Botucatu Cient\u00edfica. 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K.","year":"1999","journal-title":"Cytogenetics and Cell Genetics"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000103166"},{"issue":"3","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.ympev.2008.05.029","article-title":"Phylogenetic Relationships Within Passerida (Aves: Passeriformes): A Review and a New Molecular Phylogeny Based on Three Nuclear Intron Markers","volume":"48","author":"Johansson U. 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L.Pinheiro et\u00a0al. in press.\u201cA Cytogenetic Analysis With Chromosome Painting in Typical Antbirds (Passeriformes Thamnophilidae) and an Inference on Chromosome Evolution in Passeriformes.\u201d"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862\u2010021\u201001768\u2010y"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen\u20102025\u20100055"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1159\/000486975"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08819"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.4497"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70210","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/age.70210","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:50:15Z","timestamp":1790927415000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/age.70210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/age.70210"],"URL":"https:\/\/doi.org\/10.1002\/age.70210","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70210"}}