{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:59:30Z","timestamp":1790751570286,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/16975\u20105"],"award-info":[{"award-number":["2024\/16975\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pregnancy loss (PL) affects reproductive efficiency in beef cattle, yet its genetic architecture remains poorly understood in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bos indicus<\/jats:italic>\n                    <\/jats:styled-content>\n                    populations. Here, PL was defined as failure from pregnancy diagnosis to calving, capturing a window approximately 60\u2009days after the breeding season. This study aimed to identify genomic regions and biological pathways associated with PL in early Nellore heifers using weighted single\u2010step genome\u2010wide association study (WssGWAS). Phenotypic records from 23\u2009507 Nellore heifers were analyzed, including 17\u2009052 genotyped animals (GGP Indicus panel, Neogen) and 44\u2009233 SNPs. Genomic regions explaining more than 0.5% of additive genetic variance were used to identify putative candidate genes and perform functional enrichment analysis. The estimated heritability for PL was low (0.033), indicating strong environmental influence. WssGWAS identified 25 genomic regions across 16 chromosomes, explaining 31.16% of additive genetic variance for PL. The most relevant region was located on BTA16, explaining 6.51% of the variance, although no annotated protein\u2010coding genes were found. Additional regions occurred on BTA6, BTA14, BTA22, BTA25, and BTA29. Putative candidate genes included\n                    <jats:italic>IL4R, IL21R, LRRC4C, RBMS3, ZFPM2, PRSS23<\/jats:italic>\n                    , and\n                    <jats:italic>NDST4<\/jats:italic>\n                    , involved in immune regulation, cell adhesion, extracellular matrix remodeling, and transcriptional regulation. Functional enrichment identified Gene Ontology terms and REACTOME pathways related to nucleosome assembly, epigenetic regulation, transmembrane transport, focal adhesion, calcium signaling, immune response, and WNT signaling. These findings inform the genetic architecture of PL in Nellore cattle and suggest roles for embryo\u2013maternal communication, immune regulation, chromatin organization, and placental development in pregnancy maintenance, supporting future functional validation and genomic selection strategies.\n                  <\/jats:p>","DOI":"10.1002\/age.70212","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:23:49Z","timestamp":1790749429000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Weighted Single\u2010Step GWAS Reveals Genetic Mechanisms Underlying Pregnancy Loss in Early Nellore Heifers"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5026-9778","authenticated-orcid":false,"given":"Fl\u00e1via C.","family":"Bis","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo  Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6432-2330","authenticated-orcid":false,"given":"Nedenia Bonvino","family":"Stafuzza","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Science University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-6360","authenticated-orcid":false,"given":"Elis\u00e2ngela Chicaroni","family":"de Mattos","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo  Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7457-0792","authenticated-orcid":false,"given":"Ang\u00e9lica Simone Cravo","family":"Pereira","sequence":"additional","affiliation":[{"name":"Departamento de Nutri\u00e7\u00e3o e Produ\u00e7\u00e3o Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo  Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3874-3104","authenticated-orcid":false,"given":"Jos\u00e9 Bento Sterman","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo  Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4094-2011","authenticated-orcid":false,"given":"Fernando","family":"Baldi","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Engenharia de Alimentos Universidade de S\u00e3o Paulo  Pirassununga SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4130-2040","authenticated-orcid":false,"given":"Sabrina T.","family":"Amorim","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences Oklahoma State University  Stillwater Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/JDS.2009\u20102730"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2021.1880181"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/JAS\/SKAF326"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/JDS.2020\u201019385"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/BLOOD\u20102008\u201002\u2010142745"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/JDS.2009\u20102226"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0122874"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skag130"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23518"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.067637"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/JBG.70025"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1411000"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/WRNA.5"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297\u20109686\u201042\u20102"},{"key":"e_1_2_13_16_1","first-page":"781","volume-title":"Genomic and Personalized Medicine","author":"Conta J. 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D.Senft et\u00a0al.2022.\u201cMLL3\/MLL4 Methyltransferase Activities Control Early Embryonic Development and Embryonic Stem Cell Differentiation in a Lineage\u2010Selective Manner.\u201dbioRxiv.https:\/\/doi.org\/10.1101\/2020.09.14.296558.","DOI":"10.1038\/s41588-023-01356-4"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.1210\/EN.2018\u201000105"},{"key":"e_1_2_13_62_1","doi-asserted-by":"publisher","DOI":"10.2174\/15665240113139990072"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70212","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/age.70212","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/age.70212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:23:53Z","timestamp":1790749433000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/age.70212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":61,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/age.70212"],"URL":"https:\/\/doi.org\/10.1002\/age.70212","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70212"}}