{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:33:21Z","timestamp":1791657201858,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101663"],"award-info":[{"award-number":["82101663"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Medicine"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the relationship between icariin and the osteoblastic differentiation of vascular smooth muscle cells (VSMCs) and the signal pathway involved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We applied a universally accepted calcification model of VSMCs induced by \u03b2 glycerophosphate. Then the VSMCs calcification was observed by treatment with icariin and\/or inhibitors of estrogen receptors (ERs) and p38\u2010mitogen\u2010activated protein kinase (MAPK) signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Icariin inhibited osteoblastic differentiation and mineralization of VSMCs due to decreased ALP activity and Runx2 expression. Further study demonstrated that icariin exerted this suppression effect through activating p38\u2010MAPK but not extracellular\u2010regulated kinase, JNK or Akt. An inhibitor of p38\u2010MAPK partially reversed the inhibitory effects of icariin on osteoblastic differentiation. Interestingly, treatment of VSMCs with an ER antagonist ICI182780 and a selective ER\u03b1 receptor antagonist PPT attenuated icariin\u2010mediated inhibition effect of VSMCs calcification, associated with suppression of p38\u2010MAPK phosphorylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Icariin inhibited the osteoblastic differentiation of VSMCs, and that the inhibitory effects were mediated by p38\u2010MAPK pathways through ER\u03b1.<\/jats:p><\/jats:sec>","DOI":"10.1002\/agm2.12267","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T05:04:50Z","timestamp":1697000690000},"page":"379-385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Icariin attenuates the calcification of vascular smooth muscle cells through <scp>ER\u03b1<\/scp> \u2013 <scp>p38MAPK<\/scp> pathway"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3806-9260","authenticated-orcid":false,"given":"Jieyu","family":"He","sequence":"first","affiliation":[{"name":"Department of Geriatrics, The Second Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, The Second Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junkun","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, The Second Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8857-724X","authenticated-orcid":false,"given":"Shuang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of 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