{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:12:50Z","timestamp":1794003170252,"version":"4.8.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H04286"],"award-info":[{"award-number":["17H04286"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of Gastroent Surgery"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Peritoneal metastases (PM) frequently occur in patients with gastric cancer and result in a poor prognosis. Exosomes play pivotal roles in tumor metastasis through the transfer of microRNAs (miRNAs). We examined the exosomal miRNA profile in peritoneal fluids to identify novel biomarkers to reflect tumor burden in the peritoneum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Exosomes were isolated from peritoneal fluids of patients of gastric cancer with macroscopic (P1) or microscopic (P0CY1) peritoneal metastasis (PM) and comprehensive miRNA expression analysis was carried out. Expressions of candidate miRNAs were then validated in all 58 samples using TaqMan Advanced miRNA Assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In initial screening, we carried out comprehensive analysis of exosomal miRNA using peritoneal fluids from 11 and 14 patients with or without PM, respectively, and identified 11 dysregulated miRNAs in PM (+) samples. Validation analysis showed that four miRNAs (miR\u201021\u20105p, miR\u201092a\u20103p, miR\u2010223\u20103p, and miR\u2010342\u20103p) were significantly upregulated in 12 PM (+) samples, and their expression levels showed positive correlation with peritoneal cancer index. In contrast, miR\u201029 family were all downregulated in patients with PM (+) samples. Moreover, in 24 patients with pT4 tumor, miR\u201029 at gastrectomy tended to be lower in six patients with peritoneal recurrence with significant differences in miR\u201029b\u20103p (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.012).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Expression pattern of miRNAs in peritoneal exosomes well reflects the tumor burden in the peritoneal cavity and could be a useful biomarker in the treatment of PM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ags3.12296","type":"journal-article","created":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T08:34:22Z","timestamp":1573029262000},"page":"84-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Exosomal microRNA in peritoneal fluid as a biomarker of peritoneal metastases from gastric cancer"],"prefix":"10.1002","volume":"4","author":[{"given":"Hideyuki","family":"Ohzawa","sequence":"first","affiliation":[{"name":"Center for Clinical Research Jichi Medical University Hospital Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Kumagai","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironori","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"},{"name":"Department of Clinical Oncology Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyo","family":"Miyato","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke 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