{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T13:24:38Z","timestamp":1762608278580,"version":"3.40.5"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T00:00:00Z","timestamp":1617580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23791536"],"award-info":[{"award-number":["23791536"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of Gastroent Surgery"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Daikenchuto (TU\u2010100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU\u2010100 suppresses CPT\u201011\u2010induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU\u2010100 modulates the immune response during BT by inducing PD\u20101 expression in Peyer's patches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighteen male Wistar rats were divided into four groups: a control group; a control\u00a0+\u00a0TU\u2010100 group, given TU\u2010100 1000\u00a0mg\/kg orally for 5\u00a0d; a BT group, given CPT\u201011 250\u00a0mg\/kg intra\u2010peritoneal for 2\u00a0d; and a TU\u2010100 group, given TU\u2010100 1000\u00a0mg\/kg orally for 5\u00a0d with CPT\u201011 250\u00a0mg\/kg intra\u2010peritoneal on days 4 and 5.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0\u00a0\u00d7\u00a0104\u00a0\u00b5m<jats:sup>2<\/jats:sup> vs 29.4\u00a0\u00d7\u00a0104\u00a0\u00b5m<jats:sup>2<\/jats:sup>, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05), but improved in the TU\u2010100 group (15.4\u00a0\u00d7\u00a0104\u00a0\u00b5m<jats:sup>2<\/jats:sup>, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.005). TU\u2010100 significantly induced PD\u20101 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU\u2010100\u00a0=\u00a04.3\u00a0\u00b1\u00a04.9 vs 5.1\u00a0\u00b1\u00a010.3 vs 17.9\u00a0\u00b1\u00a017.8). The CD4<jats:sup>+<\/jats:sup> cells were increased in the BT group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) compared to the control group but decreased in the TU\u2010100 group. The Foxp3<jats:sup>+<\/jats:sup> cells were increased in the BT group compared to the control group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05), and further increased in the TU\u2010100 group compared to the BT group. CPT\u201011 significantly increased TLR4, NF\u2010\u03ba\u03b2, TNF\u2010\u03b1 mRNA expressions in the BT group. TU\u2010100 cotreatment significantly reversed these mRNA expressions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TU\u2010100 may have a protective effect against BT through PD\u20101 expression in Peyer's patch.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ags3.12460","type":"journal-article","created":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T11:08:37Z","timestamp":1617620917000},"page":"683-691","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of TU\u2010100 on Peyer's patches in a bacterial translocation rat model"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6438-2763","authenticated-orcid":false,"given":"Chie","family":"Takasu","sequence":"first","affiliation":[{"name":"Department of Surgery Institute of Health Biosciences Tokushima University Tokushima 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