{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T14:08:51Z","timestamp":1784297331864,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K09932"],"award-info":[{"award-number":["20K09932"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K17633"],"award-info":[{"award-number":["20K17633"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K18521"],"award-info":[{"award-number":["20K18521"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K08903"],"award-info":[{"award-number":["22K08903"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of Gastroent Surgery"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the impact of temporary stoma creation and its subsequent closure on gut microbiota composition and diversity in patients undergoing rectal cancer surgery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Nineteen patients with primary rectal cancer who underwent curative surgery were enrolled and divided into two groups: stoma (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910, all underwent temporary ileostomy) and non\u2010stoma (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20099). Fecal samples were collected preoperatively and 6\u2009months postoperatively. Gut microbiota composition was analyzed using 16S rRNA gene sequencing. Alpha diversity (observed operational taxonomic units and Shannon index) and beta diversity (UniFrac distances) were compared between time points. Taxonomic shift was identified using Linear discriminant analysis Effect Size (LEfSe).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the stoma group, alpha diversity significantly decreased after surgery (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.049), and beta diversity analyses revealed significant changes in microbial composition (PERMANOVA; unweighted\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.026; weighted\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.046). LEfSe analysis identified an increased abundance of potentially pathogenic genera (e.g.,\n                      <jats:italic>Enterococcus<\/jats:italic>\n                      and\n                      <jats:italic>Eggerthella<\/jats:italic>\n                      ) and a decreased abundance of short\u2010chain fatty acid\u2010producing genera (e.g.,\n                      <jats:italic>Megamonas<\/jats:italic>\n                      and\n                      <jats:italic>Anaerostipes<\/jats:italic>\n                      ). These changes persisted for at least 6\u2009months after stoma closure. In contrast, the non\u2010stoma group showed no significant alterations in microbial diversity or composition over time.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Temporary stoma creation in rectal cancer surgery induces persistent alterations in gut microbiota; these alterations are characterized by reduced diversity and a shift toward a dysbiotic profile with increased pathogenic and decreased beneficial taxa. These findings highlight the potential need for microbiota\u2010targeted strategies to mitigate long\u2010term dysbiosis in patients undergoing stoma\u2010related procedures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ags3.70094","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T07:10:02Z","timestamp":1758006602000},"page":"492-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of Pre\u2010 and Postoperative Gut Microbiota Diversity in Patients With Rectal Cancer Undergoing Stoma Creation and Closure"],"prefix":"10.1002","volume":"10","author":[{"given":"Yusuke","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of General and Gastroenterological Surgery Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Osumi","sequence":"additional","affiliation":[{"name":"Department of General, Breast and Digestive Surgery Otsu City Hospital  Otsu Shiga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0648-1370","authenticated-orcid":false,"given":"Kohei","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of General and Gastroenterological Surgery Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"},{"name":"Center for Medical Research &amp; Development, Division of Translational Research Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-5714","authenticated-orcid":false,"given":"Nahoko","family":"Kato\u2010Kogoe","sequence":"additional","affiliation":[{"name":"Department of Dentistry and Oral Surgery, Faculty of Medicine Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichi","family":"Sakaguchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control, Faculty of Medicine Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shota","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases Osaka University  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6299-3381","authenticated-orcid":false,"given":"Yoshiro","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of General and Gastroenterological Surgery Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control, Faculty of Medicine Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Ueno","sequence":"additional","affiliation":[{"name":"Department of Dentistry and Oral Surgery, Faculty of Medicine Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2540-0655","authenticated-orcid":false,"given":"Sang\u2010Woong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of General and Gastroenterological Surgery Osaka Medical and Pharmaceutical University  Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020447"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20145645"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.126516.111"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.03.004"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02777"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1110787"},{"issue":"3","key":"e_1_2_13_8_1","first-page":"246","article-title":"Postoperative Complications Are Associated With Long\u2010Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery","volume":"11","author":"Schmitt F. 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